草本植物镰刀苜蓿抗氧化活性的研究

N. N. Shvets, Yu.A. Sukhomlinov, R. Bubenchikov
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引用次数: 0

摘要

目的:研究紫花苜蓿镰刀草水、水醇和醇提取物的抗自由基和抗氧化活性。材料和方法。以俄罗斯库尔斯克地区采收的镰形苜蓿花期草为研究对象,研究了镰形苜蓿水、水醇和醇提取物的抗自由基和抗氧化活性。使用滴定法评估抗氧化活性,该方法基于紫花苜蓿镰刀草提取物中存在的还原性物质和用作滴定剂的高锰酸钾相互作用的结果。采用分光光度法研究了提取物对DPPH(2,2-二苯基-1-苦味酰肼)的灭活能力。用直接分光光度法测定了绿原酸中酚类化合物的含量,用差示分光光度法测定了芦丁中黄酮类化合物的含量。对实验所得数据进行统计分析处理。结果。对实验中获得的数据进行处理,以确定紫花苜蓿镰刀草中不同浓度的水、水-醇和醇萃取物的抗氧化和抗自由基活性水平,证实了所研究的所有类型的萃取物都存在这种效果。在以30%乙醇为萃取剂的实验条件下,其抗氧化活性最高为58.55%。以96%乙醇为萃取剂,抗氧化活性最高,为56.63%,略低,但显著高于其他指标。当乙醇用量为30%时,黄酮类化合物含量固定在最高水平(0.51%)。在相同条件下,酚类化合物的回收率为0.88%。结果表明,研究对象紫花苜蓿的抗氧化活性水平与其中酚类化合物的水平直接相关。以96%乙醇为萃取剂,提取液中羟基肉桂酸和黄酮的含量较低,分别为0.14%和0.04%。结论。对花期紫花苜蓿镰刀草进行的一系列实验结果证实了药用植物原料紫花苜蓿(Medicago falcata L.)作为一种具有抗氧化活性的原料的前景,使其有可能作为继续药理研究的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study of the antioxidant activity of the herb alfalfa sickle (Medicago falcata L.)
Objective: study of the antiradical and antioxidant activity of water, water-alcohol and alcohol extracts obtained from alfalfa sickle grass. Materials and methods. As an object of research of the antiradical and antioxidant activities of water, water-alcohol and alcohol extracts of alfalfa sickle, the grass of alfalfa sickle in the flowering stage, which was harvested in the Russian Federation in the Kursk region, was considered. The antioxidant activity was evaluated using a titrimetric method based on the result of the interaction of substances of a reducing nature that are present in extracts from alfalfa sickle grass and potassium permanganate used as a titrant. Antiradical activity was studied by spectrophotometric method based on the ability of extracts to inactivate DPPH (2,2-diphenyl-1-picrylhydrazyl). The content of phenolic compounds (direct spectrophotometry) in terms of chlorogenic acid and flavonoids (differential spectrophotometry) in terms of rutin was determined. Statistical analysis was used to process the data obtained as a result of experiments. Results. Processing of the results of data obtained during experiments to determine the level of antioxidant and antiradical activity for various concentrations of aqueous, water-alcohol and alcohol extractants from alfalfa sickle grass confirmed the presence of such effects for all types of extracts studied. Under experimental conditions with the use of 30% ethanol as an extractant, the maximum atioxidant activity was observed at the level of 58.55%. The use of 96% ethanol as an extractant showed similarly the maximum antioxidant activity, at the level of 56.63%, which is slightly less, but significantly exceeds other indicators. The content of flavonoids was fixed at the maximum level (0.51%) when using 30% ethanol. Under the same conditions, phenolic compounds were recorded with an index of 0.88%. The results obtained visualize a direct correlation between the level of antioxidant activity of the studied object of the herb Medicago falcata L. and the level of phenolic compounds in it. As for the use of 96% ethanol as an extractant, then in this extraction the content of hydroxycinnamic acids and flavonoids was less (0.14% and 0.04% respectively). Conclusion. The resulting data from a series of experiments on alfalfa sickle grass collected during the flowering phase confirm the prospects of medicinal plant raw materials Medicago falcata L. as a raw material having antioxidant activity, which makes it possible to consider it as a basis for continuing pharmacological research.
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