抗氧化剂原花青素b2 (pcb2)对体外成熟(ivm)和玻璃化应激下绵羊卵母细胞发育潜能的影响

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-03-01 DOI:10.54680/fr23210110412

Jiachen Bai, Jun Li, Longfei Wang, Shaopeng Hao, Yanhua Guo, Yucheng Liu, Zhenliang Zhang, Houru Li, Wendell Q. Sun, G. Shi, P. Wan, X. Fu

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引用次数: 0

摘要

研究表明，体外成熟(IVM)和玻璃化过程等外部应激可显著降低卵母细胞的发育能力。以往的研究表明，抗氧化剂在变化条件下的适应性获得中起着关键作用。目前，天然强效抗氧化剂PCB2在绵羊卵母细胞处理过程中对IVM和玻璃化的反应中的作用尚未探讨。目的探讨PCB2处理是否能提高体外受精和玻璃化刺激下绵羊卵母细胞的发育潜能。材料与方法实验分为两部分。首先，我们评估了PCB2对体外受精过程中卵母细胞发育的影响。IVM溶液中未添加PCB2和添加5 ug / mL PCB2作为对照组和实验组(C + 5 ug / mL PCB2)。观察IVM后卵母细胞极体挤压率(PBE)、线粒体膜电位(MMP)、ATP、活性氧(ROS)水平及早期凋亡的变化。其次，我们进一步确定PCB2是否可以改善玻璃化应激下的卵母细胞质量。比较新鲜组、玻璃化组和玻璃化后在IVM溶液中添加5 ug / mL PCB2 (V + 5 ug / mL PCB2)的卵母细胞存活率、PBE率和早期凋亡。结果与对照组相比，添加PCB2可显著提高PBE率(79.4%比62.8%，P < 0.01)和MMP水平(1.9 +/- 0.08比1.3 +/- 0.04,P < 0.01)，降低ROS水平(47.1 +/- 6.3比145.3 +/- 8.9,P < 0.01)。三磷酸腺苷(ATP)含量与细胞早期凋亡无显著性差异。与新鲜组相比，玻璃化处理显著降低卵母细胞活力(43.0% vs. 90.8%， P < 0.01)和PBE率(24.2% vs. 60.6%， P < 0.05)。然而，在成熟过程中补充5 ug / mL的pcb2对玻璃化卵母细胞的存活、PBE或早期凋亡没有影响。结论pcb2能有效拮抗体外受精过程中的氧化应激，促进卵母细胞发育。DOI: 10.54680 / fr23210110412。

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Effect of antioxidant procyanidin b2 (pcb2) on ovine oocyte developmental potential in response to in vitro maturation (ivm) and vitrification stress.

BACKGROUND It was demonstrated that external stress, such as in vitro maturation (IVM) and vitrification process can induce significantly reduced development capacity in oocytes. Previous studies indicated that antioxidants play a pivotal part in the acquisition of adaptation in changed conditions. At present, the role of the natural potent antioxidant PCB2 in response to IVM and vitrification during ovine oocyte manipulation has not been explored. OBJECTIVE To investigate whether PCB2 treatment could improve the developmental potential of ovine oocytes under IVM and vitrification stimuli. MATERIALS AND METHODS The experiment was divided into two parts. Firstly, the effect of PCB2 on the development of oocytes during IVM was evaluated. Un-supplemented and 5 ug per mL PCB2-supplemented in the IVM solution were considered as control and experimental groups (C + 5 ug per mL PCB2). The polar body extrusion (PBE) rate, mitochondrial membrane potential (MMP), ATP, reactive oxygen species (ROS) levels and early apoptosis of oocytes were measured after IVM. Secondly, we further determine whether PCB2 could improve oocyte quality under vitrification stress. The survival rate, PBE rate and early apoptosis of oocytes were compared between fresh group, vitrified group and 5 ug per mL PCB2-supplemented in the IVM solution after vitrification (V + 5 ug per mL PCB2). RESULTS Compared to the control group, adding PCB2 significantly increased PBE rate (79.4% vs. 62.8%, P < 0.01) and MMP level (1.9 +/- 0.08 vs. 1.3 +/- 0.04, P < 0.01), and decreased ROS level (47.1 +/- 6.3 vs. 145.3 +/- 8.9, P < 0.01). However, there was no significant difference in ATP content and early apoptosis. Compared to the fresh group, vitrification significantly reduced oocytes viability (43.0% vs. 90.8%, P < 0.01) as well as PBE rate (24.2% vs. 60.6%, P < 0.05). However, 5 ug per mL PCB2-supplemention during maturation had no effect on survival, PBE or early apoptosis in vitrified oocytes. CONCLUSION PCB2 could effectively antagonise the oxidative stress during IVM and promote oocyte development. DOI: 10.54680/fr23210110412.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.