黑麦异染色质。2染色体1DS衍生物的鉴定。减少了主要重复序列的数量

R. Koebner, R. Appels, K. Shepherd
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引用次数: 25

摘要

小麦背景下,小麦染色体与黑麦1R染色体长臂的同源重组表现为小麦-黑麦易位,简称1DS。1RL,已被报道。在本研究过程中,同源配对被认为受到抑制的对照群体中出现了一株具有重组表型的意外植物(308-17)。植物308-17的重组染色体携带两个位于1RL上的种子蛋白位点(即黑麦Glu-R1位点和最近发现的球蛋白样标记),但似乎缺乏c -带状异染色质。利用黑麦350家族异染色质序列的克隆探针对308-17的后代进行了进一步的研究,发现一些末端异染色质仍然存在,但数量明显减少。308-17的修饰染色体很可能不是同源重组产生的,而是某种形式的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rye heterochromatin. II. Characterization of a derivative from chromosome 1DS.1RL with a reduced amount of the major repeating sequence
Homoeologous recombination between wheat chromosomes and the long arm of chromosome 1R of rye present in a wheat background as the wheat–rye translocation, 1DS.1RL, has been reported. During the course of this study an unexpected plant (308-17), with a recombinant phenotype, arose from a control population where homoeologous pairing was thought to be suppressed. The putative recombinant chromosome in plant 308-17 carried two seed protein loci located on 1RL (namely, the rye Glu-R1 locus and a recently discovered globulin-like marker) but appeared to lack C-banded heterochromatin. Further investigation of this apparent recombinant chromosome in progeny of 308-17 using a cloned probe for the 350 family of rye heterochromatic sequence indicated that some terminal heterochromatin was still present but in a much reduced amount. Because it appears that only the terminal region has been changed, the modified chromosome in 308-17 most likely did not arise from homoeologous recombination but rather from some form ...
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