{"title":"澳大利亚肺鱼(Neoceratodus forsteri)皮肤的发育和结构与四足动物表皮适应性的关系","authors":"Lorenzo Alibardi","doi":"10.1111/azo.12462","DOIUrl":null,"url":null,"abstract":"<p>Analysis of skin development in the Australian lungfish, <i>Neoceratodus forsteri</i>, indicates that keratinocytes form 2 to 3 irregular layers in tadpoles of 2–5 cm in length and are fully stratified (7–12 layers) in juveniles of 16.5–26 cm in length. The epidermis produces mucus and mainly contains intermediate filament keratins of acidic nature but also a minute amount of neutral-basic keratins (pI 7.0–7.3), which is unique among fish. The latter keratins of 60–62 kDa, are generally present in tetrapods but the specific gene structure and amino sequence of acidic, neutral and basic keratins in <i>N. forsteri</i> are presently unknown. No other epidermal proteins are detected although biochemical data suggest the presence of non-keratin proteins, including a basic type (pI 8.0–8.2) of around 32 kDa of molecular weight. The spinulated bony layer of elasmoid scales contains glycoproteins involved in mineralization, such as osteonectin and osteopontin. Also, alkaline phosphatase, involved in calcium precipitation onto the collagen matrix, has been detected in scales of juveniles up to 26 cm in length. The present review concludes that the knowledge of the data derived from epidermal and dermal protein annotation from the recently sequenced genome of this fish species will contribute to address the study on the evolution of tetrapod skin.</p>","PeriodicalId":50945,"journal":{"name":"Acta Zoologica","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Development and structure of the skin in the Australian lungfish (Neoceratodus forsteri) in relation to epidermal adaptation of tetrapods\",\"authors\":\"Lorenzo Alibardi\",\"doi\":\"10.1111/azo.12462\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Analysis of skin development in the Australian lungfish, <i>Neoceratodus forsteri</i>, indicates that keratinocytes form 2 to 3 irregular layers in tadpoles of 2–5 cm in length and are fully stratified (7–12 layers) in juveniles of 16.5–26 cm in length. The epidermis produces mucus and mainly contains intermediate filament keratins of acidic nature but also a minute amount of neutral-basic keratins (pI 7.0–7.3), which is unique among fish. The latter keratins of 60–62 kDa, are generally present in tetrapods but the specific gene structure and amino sequence of acidic, neutral and basic keratins in <i>N. forsteri</i> are presently unknown. No other epidermal proteins are detected although biochemical data suggest the presence of non-keratin proteins, including a basic type (pI 8.0–8.2) of around 32 kDa of molecular weight. The spinulated bony layer of elasmoid scales contains glycoproteins involved in mineralization, such as osteonectin and osteopontin. Also, alkaline phosphatase, involved in calcium precipitation onto the collagen matrix, has been detected in scales of juveniles up to 26 cm in length. The present review concludes that the knowledge of the data derived from epidermal and dermal protein annotation from the recently sequenced genome of this fish species will contribute to address the study on the evolution of tetrapod skin.</p>\",\"PeriodicalId\":50945,\"journal\":{\"name\":\"Acta Zoologica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2023-04-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Zoologica\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/azo.12462\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ANATOMY & MORPHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Zoologica","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/azo.12462","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
Development and structure of the skin in the Australian lungfish (Neoceratodus forsteri) in relation to epidermal adaptation of tetrapods
Analysis of skin development in the Australian lungfish, Neoceratodus forsteri, indicates that keratinocytes form 2 to 3 irregular layers in tadpoles of 2–5 cm in length and are fully stratified (7–12 layers) in juveniles of 16.5–26 cm in length. The epidermis produces mucus and mainly contains intermediate filament keratins of acidic nature but also a minute amount of neutral-basic keratins (pI 7.0–7.3), which is unique among fish. The latter keratins of 60–62 kDa, are generally present in tetrapods but the specific gene structure and amino sequence of acidic, neutral and basic keratins in N. forsteri are presently unknown. No other epidermal proteins are detected although biochemical data suggest the presence of non-keratin proteins, including a basic type (pI 8.0–8.2) of around 32 kDa of molecular weight. The spinulated bony layer of elasmoid scales contains glycoproteins involved in mineralization, such as osteonectin and osteopontin. Also, alkaline phosphatase, involved in calcium precipitation onto the collagen matrix, has been detected in scales of juveniles up to 26 cm in length. The present review concludes that the knowledge of the data derived from epidermal and dermal protein annotation from the recently sequenced genome of this fish species will contribute to address the study on the evolution of tetrapod skin.
期刊介绍:
Published regularly since 1920, Acta Zoologica has retained its position as one of the world''s leading journals in the field of animal organization, development, structure and function. Each issue publishes original research of interest to zoologists and physiologists worldwide, in the field of animal structure (from the cellular to the organismic level) and development with emphasis on functional, comparative and phylogenetic aspects. Occasional review articles are also published, as well as book reviews.