整合子I类和II类在产生多重耐药的肺炎克雷伯菌中的流行

IF 1.1 Q3 AGRICULTURE, MULTIDISCIPLINARY

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2023-06-25 DOI:10.36103/ijas.v51i5.1739

Omar F. H., A. H. Ibrahim

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引用次数: 1

摘要

本研究旨在确定尿路感染患者分离的尿路致病性肺炎克雷伯菌中整合子I、整合子II和基因的耐药谱和分布。结果显示，150份临床标本中有137份(91%)为肺炎克雷伯菌。137株对哌拉西林和利福平的耐药率最高(94.89%)，对美罗培南的耐药率最低(40.87%)。对15株菌株的PCR扩增结果显示，对I类(intl1)和II类(intl2)整合子的比例进行了调查。分别有100% (n=15)和45% (n=7)的分离株含有intl1和intl2基因。对(5)株分离物的PCR产物进行直接测序，并用自动测序仪对两条PCR产物进行测序。分析这些序列是否存在这些基因的变异，并检测核苷酸的差异。

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THE PREVALENCE OF INTEGRON CLASS I AND II AMONG MULTI-DRUG RESISTANCE PRODUCING KLEBSIELLA pneumonia

This study aimed to determine antibiotic resistance profiles and the distribution of Integron I, Integron II, and genes among uropathogenic Klebsiella pneumoniae isolated from patients suffering from urinary tract infection. The results revealed that one hundred and thirty-seven (91%) out of 150 clinical specimens were Klebsiella pneumoniae. The isolates (137 isolates) were given a higher percentage of resistance against Piperacillin (94.89%)and Rifampicin (93.43%) and the lowest resistance was with Meropenem (40.87%). According to PCR amplification for 15 isolates showed the proportion of class I (intl1) and class II (intl2) integrons were investigated. What are 100% (n=15) and 45% (n=7) of isolates had intl1 and intl2 genes, respectively. PCR products for (Five)isolates were subjected to direct sequencing, and both strands of PCR products were sequenced with an automatic sequencer. These sequences were analyzed for the presence of variance of these genes and the detection of the differences in the nucleotides.

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来源期刊

IRAQI JOURNAL OF AGRICULTURAL SCIENCES AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

2.30

自引率

50.00%

发文量

140

审稿时长

6 weeks

期刊介绍： IRAQI JOURNAL OF AGRICULTURAL SCIENCES (IJAS)is the first agric. scientific and refereed journal established in Iraq. The first volume was published in 1966. IJAS is registered in the number 137 in 1988 of the Baghdad National Library. Years ago, it was published with one issue a year. For the time being, it is published bimonthly (6 issues for a volume). IJAS Deal with: Field Crops. Plant Breeding. Agricultural Economics. Agricultural Extension. Agricultural Mechanization. Basic sciences. Hort. Sciences. Animal Husbandry. Food Technology, Plant Pathology. Plant Entomology. Poultry Sciences. Soil Sciences. Water Resources. Veterinary. Biology.