Wistar大鼠股骨头无菌性坏死模型的建立

N. Shabaldin, A. V. Shabaldin, N. E. Popova, A. V. Postnikova, L. Bogdanov, A. Tsepokina
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引用次数: 1

摘要

的目标。目的:建立股骨头无菌性坏死动物模型,用于研究legg - calv - perthes病。材料与方法。为了诱导无菌性坏死的发生,我们使用了Wistar大鼠(n = 8),这些大鼠患有股骨头灌注不足和髋关节关节内压力增加。采用异氟醚麻醉后,我们在髋关节突出处的大腿外表面做了一个切口(约3厘米长),然后切除股骨近三分之一处的骨膜。在股骨颈周围应用致密的微静脉结扎以减少股骨头的血液灌注。进一步向髋关节腔内注射1.5 mL 2%流变葡葡萄糖溶液(10%等渗葡聚糖,30-40 kDa分子量)以增加关节内压力。大鼠于随访8周后处死,进行x线及组织学检查。我们的股骨头无菌性坏死动物模型包括legg - calv - perthes病的两个主要组成部分:关节内压力升高和股骨头血流灌注不足。所有(8/8)例患者股骨头均发生无菌性坏死。干预后8周,股骨8近端情况与印模骨折相似。我们的股骨头无菌性坏死模型充分反映了leggcalf - perthes病的发病机制,因此可以用于实验研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Modeling of femoral head aseptic necrosis in Wistar rats
Aim. To develop an animal model of femoral head aseptic necrosis for studying Legg–Calvé–Perthes disease.Materials and Methods. To induce the development of aseptic necrosis, we used Wistar rats (n = 8) which suffered from combined hypoperfusion of the femoral head and increased intra-articular pressure in the hip joint. Having employed isoflurane anesthesia, we performed an incision (≈ 3 cm length) on the outer surface of the thigh in the projection of the hip joint and then excised periosteum in the proximal third of the femur. A dense vicryl ligature was applied around the femoral neck to reduce blood perfusion of the femoral head. Further, 1.5 mL 2% rheopolyglucinum solution (10% isotonic dextran, 30-40 kDa molecular weight) was injected into the hip joint cavity to increase intra-articular pressure. Rats were sacrificed upon 8-week follow-up with subsequent X-ray and histological examination.Results. Our animal model of femoral head aseptic necrosis includes two main components of Legg–Calvé–Perthes disease: an increase in the intra-articular pressure and insufficient blood perfusion of the femoral head. In all (8/8) cases, aseptic necrosis of the femoral head was achieved. Eight weeks post intervention, the condition of the proximal femur 8 was similar to impression fracture.Conclusion. Our model of femoral head aseptic necrosis fully reflects the pathogenesis of LeggCalve-Perthes disease and can be therefore used in experimental studies.
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