伊拉克尿路感染中奇异变形杆菌部分毒力基因的分子检测

IF 1.1 Q3 AGRICULTURE, MULTIDISCIPLINARY

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2023-06-25 DOI:10.36103/ijas.v54i3.1751

R. M. S. Al-khalidy, R. A. Aburesha

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引用次数: 0

摘要

本研究集中于2020年9月至2021年1月期间，从巴格达市不同医院尿路感染患者的170份尿液样本中分离和鉴定了60株(35.2%)奇异变形杆菌。在选择性培养基上培养分离菌株，用生化反应对其进行鉴定，验证APi 20e试验。对60株分离菌进行了4种抗生素耐药性检测。结果表明，不同菌株对抗生素的耐药性存在差异。菌株对萘啶酸和氨苄西林的耐药率分别为75%和51%，而对阿米卡星和伊培南的耐药率分别为8.3%。采用PCR等分子技术对奇异变形杆菌的重要毒力因子进行了检测，发现只有18株(60%)菌株在467 bp处对rsbA呈阳性反应。在464 bp处luxS阳性27例(90%)。

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MOLECULAR DETECTION OF SOME VIRULENCE GENE IN Proteus Mirabilis ISOLATED FROM URINARY TRACT INFECTION IN IRAQ

This study was concentrated for isolation and identification of 60 (35.2%) Proteus mirabilis isolates out of 170 urine samples from patients suffering from urinary tract infection from different hospitals in Baghdad city during a period from September 2020 to January 2021. The isolates were cultivated on selective media and biochemical reactions were used to identify them confirmatory APi 20 E tests. The sixty selected isolates were tested for resistance against four antibiotics. The results shown that there were differences in the antibiotic resistance of isolates. High resistance to nalidixic acid and ampicillin were found among isolates as (75%) and (51%) respectively while the resistance of Proteus mirabilis isolates to amikacin and impenem, were(8.3%). Some important virulence factor to Proteus mirabilis was detected by using molecular techniques include PCR and it was found that only 18 (60%) of isolates gave positive result for rsbA at 467 bp. 27 (90%) of them gave positive result for luxS at 464 bp.

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来源期刊

IRAQI JOURNAL OF AGRICULTURAL SCIENCES AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

2.30

自引率

50.00%

发文量

140

审稿时长

6 weeks

期刊介绍： IRAQI JOURNAL OF AGRICULTURAL SCIENCES (IJAS)is the first agric. scientific and refereed journal established in Iraq. The first volume was published in 1966. IJAS is registered in the number 137 in 1988 of the Baghdad National Library. Years ago, it was published with one issue a year. For the time being, it is published bimonthly (6 issues for a volume). IJAS Deal with: Field Crops. Plant Breeding. Agricultural Economics. Agricultural Extension. Agricultural Mechanization. Basic sciences. Hort. Sciences. Animal Husbandry. Food Technology, Plant Pathology. Plant Entomology. Poultry Sciences. Soil Sciences. Water Resources. Veterinary. Biology.