黑米糠醇提物保护H2O2对NIH3T3细胞的细胞毒作用。

Galuh Oktavya, Y. A. Purwestri, H. T. Saragih, A. Nuriliani
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摘要

氧化应激是由线粒体中的氧代谢、辐射、药物和污染物等内源性和外源性应激源引发的,对生物系统产生负面影响。各种病理生理条件和生物体的寿命都受到这些条件的影响。在黑米等天然成分中发现的次生代谢物具有很高的抗氧化活性,可以防止氧化应激。本研究旨在考察黑米(Oryza sativa L.)乙醇提取物的效力。“Sembada Hitam”)麸皮保护h2o2诱导的NIH3T3细胞。本研究旨在探讨黑米糠提取物在H2O2诱导下对细胞毒性、细胞凋亡和细胞生长的影响。本研究采用不同浓度(50、100、150、200和300 μM)的H2O2暴露和不同浓度(7.81;15.63;31.25;62.5;125;250;500;1000 μg/mL)。结果表明,在浓度为7.81 ~ 1000 μg/mL的浓度下,暴露于50 μM和100 μM的H2O2下24 h, NIH3T3细胞的存活率维持在80%以上。这与细胞凋亡实验结果一致,表明BRB提取物对细胞凋亡有抑制作用,特别是在62.5 μg/mL和100 μM浓度下BRB与H2O2联合暴露;62.5 μg/mL和200 μM, 250 μg/mL和100 μM。此外,在BRB提取物处理下,h2o2诱导的NIH3T3细胞的生长可以维持到第5天。结果表明,62.5 μg/mL浓度预处理的白芍提取物对h2o2诱导的NIH3T3细胞具有良好的抗凋亡作用,可促进细胞增殖至第5天。综上所述,我们认为BRB提取物具有保护NIH3T3细胞免受H2O2诱导的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ethanolic Extract of Black Rice ‘Sembada Hitam’ Bran Protects the Cytotoxic Effect of H2O2 on NIH3T3 Cells.
Oxidative stress which is triggered by endogenous and exogenous stressors such as oxygen metabolism in mitochondria, radiation, drugs, and pollutants, negatively affect biological systems. Various pathophysiological conditions and the life span of organisms were affected by such condition. Secondary metabolites found in natural ingredients such as black rice have high antioxidant activity that can prevent oxidative stress. This study aimed to examine potency of the ethanolic extract of black rice (Oryza sativa L. 'Sembada Hitam') bran to protects H2O2-induced NIH3T3 cells. This research was focused to evaluate the potency of black rice bran’s (BRB’s) extract on cytotoxicity, apoptosis, and cell growth due to H2O2 induction. This study used a combination of H2O2 exposure at various concentrations (50, 100, 150, 200, and 300 μM) and BRB’s extract at various concentrations (7.81; 15.63; 31.25; 62.5; 125; 250; 500; and 1000 μg/mL). Our results showed that BRB’s extract at the concentration of 7.81 to 1000 μg/mL maintained NIH3T3 cells viability above 80% against 50 and 100 μM H2O2 exposure for 24 hours. These were in line with the apoptosis test results, which showed that the BRB’s extract suppressed apoptosis, especially the combination of BRB and H2O2 exposure at 62.5 μg/mL and 100 μM; 62.5 μg/mL and 200 μM, as well as 250 μg/mL and 100 μM, respectively. Moreover, the H2O2-induced NIH3T3 cells’ growth was maintained up to the fifth day under the BRB’s extract treatment. The result proved that pretreatment of BRB’s extract at the concentration of 62.5 μg/mL is highly effective as an anti-apoptotic and increases cell proliferation up to the fifth day on H2O2-induced NIH3T3 cells. Collecting all the results together, we suggested that BRB’s extract have a protective effect by maintaining NIH3T3 cell viability against H2O2 induction.
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