千叶跟头水、乙醇、甲醇、丙酮和氢乙醇提取物对标准克雷伯氏菌、化脓性链球菌和口腔细菌的体外抑菌作用

Negar Fallah, S. Namazi, N. Balmeh, S. Mahmoudi, Fereshteh Mirzaei Poor
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引用次数: 1

摘要

到目前为止,人们已经进行了大量的尝试来寻找抗菌化合物。本研究还探讨了阿喀琉叶对标准克雷伯氏菌、化脓性葡萄球菌和口腔细菌的抑菌作用。材料与方法:以千年木犀的地上部分为材料,分别制备水提物、乙醇提物、甲醇提物、丙酮提物和氢乙醇提物。制备克雷伯氏菌、化脓性葡萄球菌和口腔细菌标准菌株,并用Millipore滤器对提取液进行灭菌,以最低抑菌浓度(MIC)、最低杀菌浓度(MBC)和浓度为50 mg/ml的孔扩散评价提取液对上述微生物的抑菌效果。对每种细菌重复测试三次。结果:千叶跟头水提物对所有菌株的抑菌效果最明显,甲醇提物仅对化脓性葡萄球菌有抑菌作用。有效提取物的MIC和MBC均为基础浓度(50mg/ml),其余连续稀释均未见无生长带。结论:千叶阿喀琉叶可作为一种抗菌中药。综上所述,经体外药效评价,千叶阿喀琉叶可作为常规化学药物的替代药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In-vitro Antibacterial Effects of Aqueous, Ethanolic, Methanolic, Acetone and Hydro Ethanolic Extracts of Achillea Millefolium on Standard Klebsiella, S. pyogenes, and Oral Bacterias Strains
Introduction: So far, a lot of attempts have been carried out to find antimicrobial compounds. In this study, it was also tried to investigate the antibacterial effects of Achillea millefolium on standard Klebsiella, S. pyogenes, and oral bacterias strain. Material and Methods: The aerial parts of Achillea millefolium were used and the aqueous, ethanolic, methanolic, acetone and hydroethanolic extracts were prepared. After the preparation of standard strains of Klebsiella, S. pyogenes, and oral bacterias and sterilization of extracts by the Millipore filter, the antibacterial effects of these extracts on the mentioned microorganisms were assessed by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and well diffusion at the concentration 50 mg/ml. The test was repeated three times for each bacterium. Results: It was exposed that aqueous extract of Achillea millefolium had the most distinguished antimicrobial effects against all studied strains and methanolic extract had antimicrobial effects only on S. pyogenes. MIC and MBC of effective extracts were the basic concentration (50mg/ml), and non-growth zone was not observed in other serial dilution in case of all bacteria. Conclusion: The Achillea millefolium can be admitted as an antibacterial medicinal herb. Thus, it can be concluded that after evaluating their effects in vitro, Achillea millefolium can be utilized as an alternative to the routine chemical drugs.
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