The effect of combaine administration of L-arginine and omega-3 polyunsaturated fatty acids on the spectrum of free amino acids and biogen amines in hyppocampus of rats undergoing subtotal cerebral ischemia

The aim of this study was to estimate the changes in the pool of free amino acids and their derivatives in hippocampus of rats undergoing subtotal cerebral ischemia (SCI) and treated with L-arginine and omega-3 polyunsaturated fatty acids (PUFA). Experiment was held on 18 rats: 12 animals were undergoing bilateral filament occlusion of arteries carotid, 6 of them L-arginine and omega-3 PUFA was administrated. The drug omega-3 PUFA "Omegamed" (at a dose of 5 g/kg of body weight) was injected intragastrically during the week preceding the simulation of SIMG. L-arginine (at a dose of 100 mg/kg body weight) was injected intravenously just before ligation of the common carotid arteries. The analyses of free amino acids and their derivates levels in the blood plasma extracts were carried out by reversed phase HPLC. In the hippocampus of rats with SIGM, there was an increase in the levels of histidine, 3-methylhistidine, glutamine, α--aminobutyrate, isoleucine, leucine, valine, as well as a decrease in the levels of threonine, tyrosine, and α--aminoadipic acid. Administration of L-arginine and omega-3 PUFAs prevented ischemia-induced disruption of threonine, histidine, glutamine, α--aminobutyrate, α--aminoadipic acid levels, and also had a corrective effect on the serotonin system of the hippocampus.