富勒烯醇的抗氧化活性。体外生物发光监测

A.S. Sachkova , E.S. Kovel , A.A. Vorobeva , N.S. Kudryasheva
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引用次数: 8

摘要

分离酶的生物发光由于其简单的生理参数-光强度的配准,是生物传感器发展的一个前景广阔的现象。基于酶的生物发光试验被广泛用于评价生化毒性的降低。此外,酶法还可用于氧化毒性的直接生化监测。本研究考虑了富勒烯醇、富勒烯的水溶性多羟基衍生物和前景药物制剂在氧化型- K3[Fe(CN)6]和1,4-苯醌模型无机和有机毒物溶液中的抗氧化性能。两种富勒烯醇制剂:C60O2-4(OH)20-24和两种富勒烯醇C60O2-4(OH)20-24+C70O2-4(OH)20-24的混合物。基于酶的分析显示了解毒过程的特殊性:超低浓度的富勒烯醇具有活性(约10-17-10-5g/L);脱毒效率与富勒烯醇浓度无单调关系,有机氧化剂溶液的脱毒效果优于无机氧化剂溶液。高稀释富勒烯醇溶液的抗氧化作用归因于激效效应;在低剂量暴露下,解毒与刺激适应性细胞反应有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidant Activity of Fullerenols. Bioluminescent Monitoring in vitro

Bioluminescence of isolated enzymes is a perspective phenomenon for biosensors development due to simplicity of registration of a physiological parameter - light intensity. Enzyme-based bioluminescent assay is widely used to evaluate a decrease in biochemical toxicities. Also the enzyme-based assay is used for the direct biochemical monitoring of oxidative toxicity. This work considers antioxidant properties of fullerenols, water-soluble polyhydroxylated derivatives of fullerenes and perspective pharmaceutical agents, in solutions of model inorganic and organic toxicants of oxidative type – K3[Fe(CN)6] and 1,4-benzoquinone. Two fullerenol preparations were used: C60O2-4(OH)20-24 and mixture of two types of fullerenols C60O2-4(OH)20-24+C70O2-4(OH)20-24. The enzyme-based assays showed the peculiarities of the detoxification processes: ultralow concentrations of fullerenols were active (ca 10-17-10-5g/L); no monotonic dependence of detoxification efficiency on fullerenol concentrations was observed, and detoxification of organic oxidizer solutions was more effective than that of the inorganic oxidizer. The antioxidant effects of highly diluted fullerenol solutions were attributed to hormesis phenomenon; the detoxification was concerned with stimulation of adaptive cellular response under low-dose exposures.

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