利用微生物群生产高纯度低聚半乳糖(GOS)的新工艺

Rengarajan Saravanan, Shajahan Shubethar, S. Narayanan, M. Jain, S. Lade, D. Jadhav, Palamalai Maheswaran, Uday K Avalakki, A. Dubey
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引用次数: 8

摘要

半乳糖低聚糖(Galactooligosaccharides, GOS)是一种不易消化的膳食纤维,通过促进肠道内益生菌的生长对人体健康具有有益作用。此外,据报道,食用低聚糖还具有其他健康益处,如促进肠道蠕动、预防结肠癌、矿物质吸收以及防止某些致病性细菌感染。本研究的目标是开发一种高效的生物转化系统,利用微生物联合体生产≥85%纯度的GOS,并在重复的生物转化循环中重复利用细胞生物量。研究了以单孢菌MTCC 5491全细胞为β-半乳糖苷酶来源,利用酵母分离物(NUTIDY007)利用单糖生产GOS的乳糖转半乳糖基化反应。为了提高GOS的纯度,研究了GOS在全细胞中转半乳糖基化的生长和生物转化参数。在微滤膜反应器中进行了GOS的连续生产研究。单次培养获得的GOS纯度最高可达42%。在优化的条件下,单次培养最多可获得56%的GOS纯度。在反应混合物中加入第二次培养以利用葡萄糖,使GOS纯度从56%显著提高到≥85%。该产品由三至五半乳糖低聚糖组成。三糖是反应混合物的主要成分。在此条件下,产率最高可达10.9 g/L/hr。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A novel process for the production of high-purity galactooligosaccharides (GOS) using consortium of microbes
ABSTRACT Galactooligosaccharides (GOS) are nondigestible dietary fibers which have a beneficial effect on human health by promoting the growth of probiotic bacteria in the gut. In addition, other health benefits have been reported from oligosaccharides consumption such as stimulation of intestinal mobility, colon cancer prevention, mineral absorption as well as protection against certain pathogenic bacterial infections. The goal of this research was to develop an efficient biotransformation system using a consortium of microbes for the production of ≥85% pure GOS and reusing the cell biomass in repeated cycles of biotransformation. Production of GOS by lactose transgalactosylation using whole cells of Sporobolomyces singularis MTCC 5491 as a source of β-galactosidase and monosaccharides utilization by yeast isolate (NUTIDY007) were studied. For increasing the purity of GOS, growth and bioconversion parameters on the transgalactosylation by the whole cells were investigated. Further, continuous production of GOS was studied in a reactor with microfiltration membrane system. A maximum GOS purity of 42% was achieved using single culture of S. singularis. Under optimized conditions, single culture of S. singularis produced a maximum of 56% pure GOS. Addition of second culture to the reaction mixture for utilization of glucose significantly increased the GOS purity from 56% to ≥85%. The product consisted of tri- to penta-galactooligosaccharides. Trisaccharides were the main component of the reaction mixture. A maximum productivity of 10.9 g/L/hr was obtained under the optimum conditions.
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