{"title":"毛纤毛虫中儿茶素的分离、表征及植物化学筛选、分析方法的建立及RP - HPLC测定方法的验证","authors":"Ndvr Saradhi, N. Gopal, C. Chetty, K. Sushmitha","doi":"10.20902/ijptr.2019.130203","DOIUrl":null,"url":null,"abstract":"Ayurveda, an ancient system of medicines detailing a number of medicinal plants and their activities in human or animals. The present research work was aimed to develop an analytical procedure for the determination of catechins in the selected plant B.Ciliata. It is famously known as stone flower/ stone breaker having various biological activities like anti urolithiatic, antiviral, antidiabetic antitumor and cardio protective activity. The methanolic extract of the plant is isolated and a method is developed by using RP HPLC for the determination of catechins in the crude plant extract using a C18 column (200*4.6mm, 5μ) and detected at 241 nm. The method is validated for its system suitability, Linearity, Accuracy, Precision, Robustness and sensitivity as per the ICH guidelines Q2(R1) to meet the analytical procedure in academic and industrial usage 19.","PeriodicalId":14252,"journal":{"name":"International Journal of PharmTech Research","volume":"19 1","pages":"18-24"},"PeriodicalIF":0.0000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation, Characterization & Phytochemical Screening,\\nAnalytical Method Development and Validation for the\\nDetermination of Catechins in B.ciliata by RP HPLC Method\",\"authors\":\"Ndvr Saradhi, N. Gopal, C. Chetty, K. Sushmitha\",\"doi\":\"10.20902/ijptr.2019.130203\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Ayurveda, an ancient system of medicines detailing a number of medicinal plants and their activities in human or animals. The present research work was aimed to develop an analytical procedure for the determination of catechins in the selected plant B.Ciliata. It is famously known as stone flower/ stone breaker having various biological activities like anti urolithiatic, antiviral, antidiabetic antitumor and cardio protective activity. The methanolic extract of the plant is isolated and a method is developed by using RP HPLC for the determination of catechins in the crude plant extract using a C18 column (200*4.6mm, 5μ) and detected at 241 nm. The method is validated for its system suitability, Linearity, Accuracy, Precision, Robustness and sensitivity as per the ICH guidelines Q2(R1) to meet the analytical procedure in academic and industrial usage 19.\",\"PeriodicalId\":14252,\"journal\":{\"name\":\"International Journal of PharmTech Research\",\"volume\":\"19 1\",\"pages\":\"18-24\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of PharmTech Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.20902/ijptr.2019.130203\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of PharmTech Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20902/ijptr.2019.130203","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Isolation, Characterization & Phytochemical Screening,
Analytical Method Development and Validation for the
Determination of Catechins in B.ciliata by RP HPLC Method
Ayurveda, an ancient system of medicines detailing a number of medicinal plants and their activities in human or animals. The present research work was aimed to develop an analytical procedure for the determination of catechins in the selected plant B.Ciliata. It is famously known as stone flower/ stone breaker having various biological activities like anti urolithiatic, antiviral, antidiabetic antitumor and cardio protective activity. The methanolic extract of the plant is isolated and a method is developed by using RP HPLC for the determination of catechins in the crude plant extract using a C18 column (200*4.6mm, 5μ) and detected at 241 nm. The method is validated for its system suitability, Linearity, Accuracy, Precision, Robustness and sensitivity as per the ICH guidelines Q2(R1) to meet the analytical procedure in academic and industrial usage 19.
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