酶基和组织基膜电极的研究进展

L. Macholán
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引用次数: 7

摘要

本文综述了本实验室主要以CLARK氧传感器为基础设计的与交联氧化酶相关的11种电流型三苯酶电极的工作。特别关注了与植物组织切片耦合的传感器用于l -抗坏血酸和酚类物质的测定。报道了一种“第二代”双酶电极,它使形成代谢偶对的两种分析物能够同时测定。本文还介绍了第一个用于测定尿素和精氨酸酶的电导酶电极模型的原理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Recent progress in developing enzyme and tissue‐based membrane electrodes
The article summarizes the work of this laboratory on the design of eleven types of amperometricpenzyme electrodes mainly based on the CLARK oxygen sensor in connection with cross-linked oxidases. Special attention is paid to the sensors coupled with plant tissue slice for determining L-ascorbic acid and phenols. A “second generation” bienzyme electrode is reported which enables tow analytes forming a metabolic couple to be determined simultaneously. A principle of the first useful model of conductimetric enzyme electrode for assaying urea and arginase is also presented.
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