{"title":"星形胶质细胞和小胶质细胞对减压病兔脊髓损伤的影响","authors":"Cui-cui Wang, Xiaohong Liu, Yong-cheng Cao, L. Bi","doi":"10.3760/CMA.J.ISSN.1007-6239.2015.02.013","DOIUrl":null,"url":null,"abstract":"Objective \nTo investigate the effects of astrocyte and microglia on spinal cord injury of the rabbits in decompression sickness (DCS). \n \n \nMethods \nTwenty-one healthy adult male New Zealand rabbits were averagely divided into 3 groups according to random number table: control group, safety relief group and DCS group. Experimental animals were placed in the pressure cabin. Safety relief group model referred to the Chinese Navy diving decompression tables when decompression applied. In DCS group, the pressure equably was increased to 0.8 MPa (absolute pressure) within 5 min by compressed air, maintained for 60 min and then equably decompressed to normal pressure within 5 min. The change of pathology morphology in the spinal cord of thoracolumbar vertebra was observed by light microscope. The expression of tumor necrosis factor-α (TNF-α) and Glial Fibrillary Acidic Protein (GFAP) mRNA were measured by real-time polymerase chain reaction (real-time PCR) and the expression of TNF-α, GFAP, ionized calcium binding adapter molecule 1 (IBA1) protein was measured by immunohistochemistry method. \n \n \nResults \nThere were some cavity formations on white matter of spinal cord in DCS group. The relative expression of TNF-α mRNA was higher in DCS (6.28±1.73) than that in control group (1.00±0.14) and in safety relief group (1.34±0.42) (P<0.01). The relative expression of GFAP mRNA was higher in DCS (7.39±2.04) than in that control group (1.02±0.26) and in safety relief group (1.63±0.90) (P<0.01). The expression of TNF-α protein was significantly higher in DCS group (24.14±2.61) than that in control group (6.71±1.25) and in safety relief group (8.28±1.11) (P<0.01). The expression of GFAP protein was also significantly higher in DCS (18.20±4.38) than that in control group (4.30±2.70) and in safety relief group (6.20±2.92) (P<0.01). The expression of IBA1 protein was also significantly higher in DCS (21.53±1.37) than that in control group (5.94±0.36) and in safety relief group (6.69±0.81) (P<0.01). \n \n \nConclusions \nThe astrocyte and microglia may play a key role in pathophysiology of spinal cord injury. They are activated in the early stage of DCS and accompanied with massive release of TNF-α, which causes excessive inflammation reaction and induces the spinal cord injury finally. \n \n \nKey words: \nDecompression sickness; Spinal cord injuries; Astrocytes; Microglia; Tumor necrosis factor-alpha","PeriodicalId":9904,"journal":{"name":"Chinese Journal of Aerospace Medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2015-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of astrocyte and microglia on spinal cord injury of the rabbits in decompression sickness\",\"authors\":\"Cui-cui Wang, Xiaohong Liu, Yong-cheng Cao, L. Bi\",\"doi\":\"10.3760/CMA.J.ISSN.1007-6239.2015.02.013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective \\nTo investigate the effects of astrocyte and microglia on spinal cord injury of the rabbits in decompression sickness (DCS). \\n \\n \\nMethods \\nTwenty-one healthy adult male New Zealand rabbits were averagely divided into 3 groups according to random number table: control group, safety relief group and DCS group. Experimental animals were placed in the pressure cabin. Safety relief group model referred to the Chinese Navy diving decompression tables when decompression applied. In DCS group, the pressure equably was increased to 0.8 MPa (absolute pressure) within 5 min by compressed air, maintained for 60 min and then equably decompressed to normal pressure within 5 min. The change of pathology morphology in the spinal cord of thoracolumbar vertebra was observed by light microscope. The expression of tumor necrosis factor-α (TNF-α) and Glial Fibrillary Acidic Protein (GFAP) mRNA were measured by real-time polymerase chain reaction (real-time PCR) and the expression of TNF-α, GFAP, ionized calcium binding adapter molecule 1 (IBA1) protein was measured by immunohistochemistry method. \\n \\n \\nResults \\nThere were some cavity formations on white matter of spinal cord in DCS group. The relative expression of TNF-α mRNA was higher in DCS (6.28±1.73) than that in control group (1.00±0.14) and in safety relief group (1.34±0.42) (P<0.01). The relative expression of GFAP mRNA was higher in DCS (7.39±2.04) than in that control group (1.02±0.26) and in safety relief group (1.63±0.90) (P<0.01). The expression of TNF-α protein was significantly higher in DCS group (24.14±2.61) than that in control group (6.71±1.25) and in safety relief group (8.28±1.11) (P<0.01). The expression of GFAP protein was also significantly higher in DCS (18.20±4.38) than that in control group (4.30±2.70) and in safety relief group (6.20±2.92) (P<0.01). The expression of IBA1 protein was also significantly higher in DCS (21.53±1.37) than that in control group (5.94±0.36) and in safety relief group (6.69±0.81) (P<0.01). \\n \\n \\nConclusions \\nThe astrocyte and microglia may play a key role in pathophysiology of spinal cord injury. They are activated in the early stage of DCS and accompanied with massive release of TNF-α, which causes excessive inflammation reaction and induces the spinal cord injury finally. \\n \\n \\nKey words: \\nDecompression sickness; Spinal cord injuries; Astrocytes; Microglia; Tumor necrosis factor-alpha\",\"PeriodicalId\":9904,\"journal\":{\"name\":\"Chinese Journal of Aerospace Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-06-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Journal of Aerospace Medicine\",\"FirstCategoryId\":\"1087\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.1007-6239.2015.02.013\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Journal of Aerospace Medicine","FirstCategoryId":"1087","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1007-6239.2015.02.013","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effects of astrocyte and microglia on spinal cord injury of the rabbits in decompression sickness
Objective
To investigate the effects of astrocyte and microglia on spinal cord injury of the rabbits in decompression sickness (DCS).
Methods
Twenty-one healthy adult male New Zealand rabbits were averagely divided into 3 groups according to random number table: control group, safety relief group and DCS group. Experimental animals were placed in the pressure cabin. Safety relief group model referred to the Chinese Navy diving decompression tables when decompression applied. In DCS group, the pressure equably was increased to 0.8 MPa (absolute pressure) within 5 min by compressed air, maintained for 60 min and then equably decompressed to normal pressure within 5 min. The change of pathology morphology in the spinal cord of thoracolumbar vertebra was observed by light microscope. The expression of tumor necrosis factor-α (TNF-α) and Glial Fibrillary Acidic Protein (GFAP) mRNA were measured by real-time polymerase chain reaction (real-time PCR) and the expression of TNF-α, GFAP, ionized calcium binding adapter molecule 1 (IBA1) protein was measured by immunohistochemistry method.
Results
There were some cavity formations on white matter of spinal cord in DCS group. The relative expression of TNF-α mRNA was higher in DCS (6.28±1.73) than that in control group (1.00±0.14) and in safety relief group (1.34±0.42) (P<0.01). The relative expression of GFAP mRNA was higher in DCS (7.39±2.04) than in that control group (1.02±0.26) and in safety relief group (1.63±0.90) (P<0.01). The expression of TNF-α protein was significantly higher in DCS group (24.14±2.61) than that in control group (6.71±1.25) and in safety relief group (8.28±1.11) (P<0.01). The expression of GFAP protein was also significantly higher in DCS (18.20±4.38) than that in control group (4.30±2.70) and in safety relief group (6.20±2.92) (P<0.01). The expression of IBA1 protein was also significantly higher in DCS (21.53±1.37) than that in control group (5.94±0.36) and in safety relief group (6.69±0.81) (P<0.01).
Conclusions
The astrocyte and microglia may play a key role in pathophysiology of spinal cord injury. They are activated in the early stage of DCS and accompanied with massive release of TNF-α, which causes excessive inflammation reaction and induces the spinal cord injury finally.
Key words:
Decompression sickness; Spinal cord injuries; Astrocytes; Microglia; Tumor necrosis factor-alpha