一种新型高效液相色谱SPME热脱附界面

Journal of Analytical Sciences, Methods and Instrumentation Pub Date : 2013-10-08 DOI:10.4236/JASMI.2013.34028

J. Acevedo, Mohamed Nour Kayali Sayadi, L. M. Díez

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引用次数: 3

摘要

开发了一种新的SPME/HPLC接口。它是基于SPME纤维的热解吸和解吸后的分析物的有机溶剂收集，通过氩气将其扫入一个小的有机溶剂体积中，该有机溶剂进一步注入HPLC色谱仪。以萘、苊、芴、菲、蒽等5种不同挥发性的多环芳烃为测试化合物并进行荧光检测，研究了萃取解吸参数。回归系数接近0.99,RDS = 3)，检出限为0.07 ~ 0.99 μg。发现了L-1。采用一种测定水样中上述多环芳烃的方法。将结果与550.1 EPA法提供的结果进行比较，两种方法在0.05显著水平上一致。

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A New SPME Thermal Desorption Interface for HPLC

A new SPME/HPLC interface is developed. It is based on thermal desorption from the SPME fiber and organic solvent collection of the desorbed analytes by sweeping them with an argon flow into a small organic solvent volume which is further injected into the HPLC chromatograph. Extraction and desorption parameters were investigated using five PAHs of different volatilities (naphthalene, acenaphthalene, fluorene, phenanthrene and anthracene) as test compounds and fluorimetric detection. Regression coefficients closed to 0.99 with RDS = 3) and detection limits in the range 0.07 - 0.99 μg.L-1 were found. A method was applied to determine the above PAHs in water samples. The results were compared with those supplied by the 550.1 EPA method showing the agreement of both methods at the 0.05 significance level.

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Journal of Analytical Sciences, Methods and Instrumentation

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