响应面法优化亚洲积雪草芽化及愈伤组织培养基标准化

K. Gururajan, D. Hegde, Sharadhi Udupa, Shobith Shetty, P. Ujwal
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引用次数: 1

摘要

积雪草(Centella asiatica)是一种用作食品补充剂的草药,在传统和民间医学中使用,因其治疗特性而被过度开发。缺乏适当的栽培和替代方法可能导致野生品种的枯竭。植物组织培养技术的应用有助于几种植物的离体再生和保存。本研究报道了利用田间栽培植物保护积雪草的培养基标准化和愈伤组织诱导。在愈伤组织诱导的培养基标准化中,添加2 mg的全强度MS (Murashige和Skoog)培养基获得了可观的结果。L-1 IAA(吲哚乙酸)和0.5 mg的混合物。l - 1kn (Kinetin), 1mg。L-1 IAA,而MS培养基中添加0.5 mg的组合。l - 1kn, 1.5 mg。L-1 IAA用于愈伤组织增殖。6-苄基氨基嘌呤(6- Benzyl amino purine, BAP)与IAA的组合在微繁试验中显示出较高的出苗频率,并利用响应面法(Response surface methodology, RSM)对其进行了进一步优化,以蔗糖和孵育时间为参数,研究了它们的个体效应和交互效应。0.5 mg BAP和IAA处理13 d后,芽高达到3.8 cm。L-1和蔗糖浓度为6%,R2值为0.73。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
OPTIMIZATION OF SHOOT INITIATION USING RESPONSE SURFACE METHODOLOGY AND CALLUS MEDIA STANDARDIZATION FOR MICROPROPAGATION OF CENTELLA ASIATICA
Centella asiatica, a medicinal herb consumed as a food supplement and used in traditional and folklore medicine is being overexploited for its therapeutic properties. Lack of adequate cultivation and replacement practices could engender the depletion of the wild variety. Application of plant tissue culture has helped in the regeneration and preservation of several plants in-vitro. The present study reports media standardization for micropropagation and callus induction for the conservation of Centella asiatica using field-grown plants. In media standardization for callus induction, appreciable results were obtained where full strength MS (Murashige and Skoog) media was supplemented with 2 mg.L-1 IAA (Indole acetic acid) and a combination of 0.5 mg.L-1 Kn (Kinetin) with 1 mg.L-1 IAA while MS media supplemented with a combination of 0.5 mg.L-1 Kn and 1.5 mg.L-1 IAA was used for callus proliferation. Combination of BAP (6- Benzyl amino purine) and IAA from preliminary experimentation on micropropagation showed high shooting frequency and were further optimized with parameters such as sucrose and incubation period using Response surface methodology (RSM) to study their individual and interactive effects. A maximum shoot height of 3.8 cm was obtained from the optimized values after 13 days with both BAP and IAA at 0.5 mg.L-1 and sucrose at 6% with the R2 value at 0.73.
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