红曲霉生产无柑桔素红曲霉素深层发酵培养基的优化

Di Chen, Yuan Xue, Mian-hua Chen, Zhenjing Li, Chang-lu Wang
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引用次数: 22

摘要

微生物发酵生产不含柠檬酸的红曲霉色素有利于食品工业的发展。本研究研究了碳源、氮源和无机盐对红曲霉M9细胞生长、红曲霉素(monascin, MS)和柑桔素(citrinin, CT)产生的影响。为使无柠檬黄蛋白MS在深层培养中产量最大化,确定了50 g/L粳米粉、20 g/L NH4NO3、3 g/L NaNO3、1.5 g/L KH2PO4、1 g/L MgSO4·7H2O、0.2 g/L MnSO4的培养基组成。在此条件下,高效液相色谱法检测不到CT。MS的产率达到14.11 mg/g,比优化前提高了约30%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of submerged fermentation medium for citrinin-free monascin production by Monascus
ABSTRACT Microbial fermentation of citrinin-free Monascus pigments is in favor in the development of food industry. This study investigated the influences of carbon source, nitrogen source, and mineral salts on the cell growth, monascin (MS), and citrinin (CT) production in Monascus M9. A culture medium composition was established for maximizing the production of citrinin-free MS in submerged culture, as follows: 50 g/L Japonica rice powder, 20 g/L NH4NO3, 3 g/L NaNO3, 1.5 g/L KH2PO4, 1 g/L MgSO4 · 7H2O, 0.2 g/L MnSO4. Under these conditions, no CT was detectable by high performance liquid chromatography. The yield of MS reached 14.11 mg/g, improving approximately 30% compared with before optimization.
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