高选择性和灵敏度的DNA测量微电阻抗谱

The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. Pub Date : 2005-06-05 DOI:10.1109/SENSOR.2005.1497322

Sang Wook Lee, T. Yamamoto, Teruo Fujii

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引用次数: 2

摘要

研制了一种新型的/spl μ /-EIS(微电阻抗谱)装置，并进行了实验验证。/spl mu/-EIS系统的特点是不仅可以不加标记地检测DNA，而且可以控制增强EIS信号的构象。/spl mu/-EIS系统完全集成了由聚二甲基硅氧烷(PDMS)，微测量电极和微泵组成的微流控网络，以便通过外部PC系统控制进行半自动测量。微EIS系统能够在体积为几pl的水溶液中检测到几nM的DNA浓度，这是由于其构象增强阻抗响应。作为构象变化诱导DNA分析的第一个演示，我们评估了不同长度和浓度的DNA的频率和电场强度依赖性。

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Highly selective and sensitive DNA measurement by microelectrical impedance spectroscopy

A novel /spl mu/-EIS (microelectrical impedance spectroscopy) device for label free analysis of DNA are developed and experimentally demonstrated. The feature of /spl mu/-EIS system is not only detecting DNA without any labelling, but also controlling the conformation that would enhance the EIS signal. The /spl mu/-EIS system is fully integrated with a microfluidic network made of polydimethylsiloxane (PDMS), a micromeasurement electrode and a micropump in order to conduct a semiautomated measurement controlled by an external PC system. The micro EIS system is capable of detecting the DNA concentration of a few nM in aqueous solution of a few pl in volume by virtue of the conformation enhanced impedance response. As a first demonstration of conformational change induced DNA analysis, the frequency and the electric field strength dependence of various length and concentrations of DNA were evaluated.

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来源期刊

The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05.

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