使用紫外荧光进行连续、快速的生物气溶胶检测:室外测试结果

J. Eversole, J. Hardgrove, W. Cary, D. P. Choulas, M. Seaver
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引用次数: 41

摘要

本报告描述了一种仪器的设计和操作原理,用于分别监测环境气溶胶的生物和非生物成分浓度。还描述了一种室外测试方法和最近的结果。单粒子荧光分析仪(SPFA)通过采样喷嘴将周围空气吸入光学室。每个气溶胶粒子的探测信号包括弹性散射响应和两个不同光谱波段的荧光发射。生物气溶胶的区分是基于在266纳米波长激光脉冲激发后测量的粒子的紫外线(UV)荧光的相对强度。在室外试验中,两种类型的生物以气溶胶喷雾剂的形式释放:厄尔温菌除草剂营养细胞和枯草芽孢杆菌孢子。SPFA参加了总共40项户外测试中的39项。27个试验释放出除草剂芽孢杆菌,9个试验释放出枯草芽孢杆菌。结果表明,该方法对草芽孢杆菌和枯草芽孢杆菌的检测可靠性分别为90%和87%,灵敏度接近1粒/l。一些测试涉及释放非生物气溶胶,这不会导致仪器中的荧光信号发生重大变化。©1999 John Wiley & Sons, Inc.* Field analyst Chem technology 3: 249 - 259,1999
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Continuous, rapid biological aerosol detection with the use of UV fluorescence: Outdoor test results
This report describes the design and principles of operation of an instrument for separately monitoring ambient aerosol concentrations of biological and nonbiological composition. A method of outdoor testing, and recent results, are also described. The single-particle fluorescence analyzer (SPFA) draws ambient air into an optical chamber through a sampling nozzle. Detection signals from each aerosol particle consist of an elastic scattering response as well as fluorescent emission in two distinct spectral bands. Differentiation of biological aerosols is based on the relative intensity of ultraviolet (UV) fluorescence measured from the particles following excitation with a 266-nm-wavelength laser pulse. During outdoor tests two types of organisms were released as aerosol sprays: Erwinia herbicola vegetative cells and Bacillus subtilis spores. The SPFA participated in 39 of a total set of 40 outdoor tests. Twenty-seven tests released E. herbicola and nine released B. subtilis. Analysis of these results indicates that a reliability of detection of about 90% for E. herbicola, and of about 87% for B. subtilis were achieved under the conditions of the tests, with a sensitivity near 1 particle/l. Some tests involved release of nonbiological aerosols that did not result in a significant change in fluorescent signals in the instrument. © 1999 John Wiley & Sons, Inc.* Field Analyt Chem Technol 3: 249–259, 1999
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