Detection of ESBL producing E.coli and Klebsiella spp in urinary tract infection due to long exposure of catheterize condition in Covid positive patient admitted in HDU

Long exposure of broad spectrum antibiotic can severe causes of extended-spectrum beta-lactamase (ESBL) production within bacteria in UTI patient. Community-acquired infections due to ESBL producing and both are rising worldwide. To assess the prevalence and antibiotics resistance patterns of ESBL-producing and Klebsiella spp. from covid positive patient admitted in HDU with catheterized condition. A hospital-based cross-sectional study was conducted on Salboni Super speciality Hospital (Covid Level-IV) at West Bengal, India from August 2020 to August2021and a total of 1116 urine samples were collected from catheterized patient admitted in male and female HDU due to exposure of covid. Samples were cultured on CLED agar and then Identification of the isolates was done by Gram's staining followed by biochemical tests. Antibiotic susceptibility testing was done by modified Kirby-Bauer disc diffusion method and interpretation was done following Clinical and Laboratory Standard Institute (CLSI) guidelines foe detection of ESBL producing and . present study showed 51.6% (n-326) and 37.2% (n-235) Klebsiella spp. from 631 significant growths from both male and female patients. Out of this, total ESBL producing and spp. seen 55% (n-309) from both male and femle patient in different age group. Finally Isolated ESBL producing i 45.9 % (n-142) and ESBL producing Klebsiella spp. 54 % (n- 167) from both male and female HDU.Detection of ESBL producing and due to prolonged uses of broad spectrum antibiotic vigorously. The occurrence of multidrug resistance to the third-generation cephalosporins, aminoglycosides, fluoroquinolones, trimethoprim-sulfamethoxazole, and tetracyclines is more common among ESBL producers. It may help to detect other organism in same condition, so that it will be importance in clinical aspect of future treatment for clinician.