土耳其地中海地区桃金娘叶和果实提取物的生物学和抗氧化能力评价

A. Gunyakti, M. Asan-Ozusaglam, Muhammad Mujtaba
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引用次数: 2

摘要

最近在人类和鱼类中,大多数引起疾病的病原微生物对某些抗菌剂产生了耐药性。这种情况造成了健康和经济问题。在此背景下,本研究旨在评价桃金娘叶和果实的抗菌和抗氧化活性。采用圆盘扩散法测定了该植物叶、果提取物对5种鱼类、7种食源性人致病菌和2种酵母菌的抗菌活性。为此,用正己烷和甲醇作为有机溶剂从干叶和干果中提取提取物。最高抑制值记录为;叶正己烷、叶甲醇和果正己烷提取物对亲水拟南蝽ATCC 19570鱼致病菌的抑制效果分别为24.59 mm、21.76 mm和23.78 mm,果甲醇提取物对粪肠拟南蝽ATCC 29212(人食源性致病菌)的抑制效果为16.61 mm。MBC和MFC结果显示,所有提取物对整个试验微生物都有抑制活性。甲醇提取物的抗氧化活性高于正己烷提取物。ic50值在162.82 ~ 633.01 μg/mL范围内,甲醇提取物的活性最高(162.82 μg/mL)。根据总抗氧化能力法,叶甲醇提取物的抗氧化活性最高,为115.91 mg AE/g。FRAP法提取的活性最高,为147.97mg TE/g。综上所述,黄芪可作为天然、经济的抗菌和抗氧化物质的候选提取物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of Biological and Antioxidant Capacities of Myrtuscommunis L. Leaf and Fruit Extracts from Mediterranean Region of Turkey
Recently in humans and fishes most of the disease causing pathogenic microorganisms developed resistance against certain antimicrobial agents. This situation is causing both health and economic problems. In this context present study was designed to evaluate the antimicrobial and antioxidant activities of leaves and fruits of Myrtuscommunis. Antimicrobial activity of the plant’s leaf and fruit extracts was tested by using disc diffusion method against five fish, seven food-borne human pathogens and twoyeast strains. For this purpose, n-hexane and methanol an organic solvent were used for the obtaining the extract from dried leaves and fruit. The highest inhibition values was recorded as; for leaf n-hexane, leaf methanol and fruit n-hexane extracts against A. hydrophila ATCC 19570 fish pathogen as 24.59 mm, 21.76 mm and 23.78 mm, respectively while for fruit methanol extract against E. faecalis ATCC 29212 (human food-borne pathogen) as 16.61 mm. MBC and MFC results revealed that all extracts showed inhibitory activity against the entire test microorganisms. Methanol extracts exhibited higher antioxidant activity than n-hexane extracts. IC 50 values were recorded in the range of 162.82-633.01 μg/mL and the highest activity was recorded for methanol extract (162.82 μg/mL). According to total antioxidant capacity method, the leaf methanol extract has been shown to have the highest activity as 115.91 mg AE/g.The highest activity (147.97mg TE/g) was found by the leaf methanol extract using FRAP method. Considering the results M. communis can be suggested as possible candidate for the extraction of natural and economic antimicrobial and antioxidant material.
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