T. Tamura, Atsushi Kataoka, Lidan Shu, Akihiko Ashida, Hidehiko Tanaka, K. Inagaki
{"title":"DNA胞嘧啶- c5 -甲基化酶抑制剂的体外筛选方法","authors":"T. Tamura, Atsushi Kataoka, Lidan Shu, Akihiko Ashida, Hidehiko Tanaka, K. Inagaki","doi":"10.1080/1057563029001/4809","DOIUrl":null,"url":null,"abstract":"A specific inhibitor of DNA cytosine C5-methylases would be useful for studying genomic imprinting, X-chromosome inactivation, carcinogenesis, and regulation of tissue-specific gene expression, for these physiological phenomena appears to be regulated through DNA methylation in promoter sequences. This paper reports a novel convenient in vitro assay method for screening DNA cytosine C5-methylase inhibitor. Our method uses a commercially available Hae III methylase (cytosine C5 methylase), its corresponding Hae III endonuclease, and u DNA as their substrate.","PeriodicalId":18873,"journal":{"name":"Natural Product Letters","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"An in vitro screening method for DNA cytosine-C5-methylase inhibitor\",\"authors\":\"T. Tamura, Atsushi Kataoka, Lidan Shu, Akihiko Ashida, Hidehiko Tanaka, K. Inagaki\",\"doi\":\"10.1080/1057563029001/4809\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A specific inhibitor of DNA cytosine C5-methylases would be useful for studying genomic imprinting, X-chromosome inactivation, carcinogenesis, and regulation of tissue-specific gene expression, for these physiological phenomena appears to be regulated through DNA methylation in promoter sequences. This paper reports a novel convenient in vitro assay method for screening DNA cytosine C5-methylase inhibitor. Our method uses a commercially available Hae III methylase (cytosine C5 methylase), its corresponding Hae III endonuclease, and u DNA as their substrate.\",\"PeriodicalId\":18873,\"journal\":{\"name\":\"Natural Product Letters\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Natural Product Letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/1057563029001/4809\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Product Letters","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/1057563029001/4809","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
An in vitro screening method for DNA cytosine-C5-methylase inhibitor
A specific inhibitor of DNA cytosine C5-methylases would be useful for studying genomic imprinting, X-chromosome inactivation, carcinogenesis, and regulation of tissue-specific gene expression, for these physiological phenomena appears to be regulated through DNA methylation in promoter sequences. This paper reports a novel convenient in vitro assay method for screening DNA cytosine C5-methylase inhibitor. Our method uses a commercially available Hae III methylase (cytosine C5 methylase), its corresponding Hae III endonuclease, and u DNA as their substrate.
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