甜菜根富含多酚的高效液相色谱标准提取物的抗氧化性能

International Journal of Research and Development in Pharmacy and Life Sciences Pub Date : 2017-04-01 DOI:10.21276/IJRDPL.2278-0238.2017.6(3).2619-2624

R. Indu, A. Adhikari, M. Ray, A. Hazra, T. Sur, A. Das

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引用次数: 3

摘要

目的:评价标准甜菜根或甜菜根的抗氧化作用。方法:采用高效液相色谱法对甜菜根冻干水提物中多酚类物质进行定量和标准化分析。通过体外生化测定，考察BR对自由基的失活作用，包括1,1-二苯基-2-苦味酰肼(DPPH)、过氧化氢和超氧自由基清除活性。测定了BR中亚硝酸盐的含量。此外，还对BR的蛋白质变性特性进行了抑制。结果:高效液相色谱分析显示，BR中含有9种生物活性多酚。此外，还注意到强剂量依赖性抗氧化性能。BR对DPPH、过氧化氢和超氧自由基的抑制浓度(IC50)分别为104.44μg/ml、4.06μg/ml和71.43μg/ml。BR中亚硝酸盐含量为3.359 mM/mg，抑制蛋白质变性(IC50为594μg/ml)。结论:甜菜根具有较强的抗氧化作用，这可能与甜菜根中多酚的存在有关。

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Antioxidant properties of polyphenolic rich HPLC standardized extract of Beta vulgaris L. roots

OBJECTIVES: The present study was planned to evaluate the antioxidant effects of standardized Beta vulgaris L. or beetroot. METHODS: The polyphenols present in lyophilized water extract of beetroot (BR) were quantified and standardized by HPLC. Deactivation of free radicals by BR was studied by in vitro biochemical assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide and superoxide radical scavenging activities. Nitrite content of BR was estimated. Moreover, inhibition of protein denaturation properties of BR was carried out. RESULTS : HPLC chromatogram revealed presence of nine bioactive polyphenols in BR. Further, strong dose-dependent antioxidant properties were noted. IC50 (Inhibitory Concentration) of BR for DPPH, hydrogen peroxide and superoxide radical were 104.44μg/ml, 4.06μg/ml and 71.43μg/ml respectively. BR also showed enriched in nitrite content 3.359 mM/mg and it inhibited protein denaturation (IC50 594μg/ml). CONCLUSION: Beetroot was found to possess strong antioxidant property that may be due to presence of polyphenols.

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International Journal of Research and Development in Pharmacy and Life Sciences

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