Brusatol抑制前列腺癌细胞生长,降低缺氧条件下HIF-1α/VEGF表达和糖酵解

Mi Wang, Liyang Dai, Wei Yan, Ying Chen, Yakun Wang
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引用次数: 0

摘要

前列腺癌(PCa)具有很高的发病率和死亡率,这促使我们寻找一种独特而有效的治疗药物。Brusatol是一种三萜降解衍生物,具有抗肿瘤活性。然而,Brusatol在前列腺癌中的作用尚未完全阐明。因此,本研究旨在探讨Brusatol对前列腺癌细胞的影响。选择DU145和PC-3细胞系作为实验模型。将Brusatol添加到相关细胞中培养后,通过CCK-8和集落形成实验评估细胞存活率;流式细胞术计算细胞凋亡率;transwell法检测细胞迁移和侵袭能力。western blotting检测Vimentin、N-cadherin、E-cadherin、occluden -1 (ZO-1)、缺氧诱导因子1α (HIF-1α)、血管内皮生长因子(VEGF)、葡萄糖转运蛋白1 (GLUT1)、己糖激酶2 (HK2)、乳酸脱氢酶(LDHA)蛋白表达,并用相关设备检测细胞葡萄糖消耗。在DU145和PC-3细胞中,Brusatol显著降低细胞增殖,促进细胞凋亡,阻碍细胞迁移和侵袭。缺氧条件下HIF-1α和VEGF蛋白水平明显降低。此外,GLUT1、HK2和LDHA的表达减少,导致葡萄糖消耗以Brusatol浓度依赖的方式减少。这些发现表明Brusatol是一种有效的抗肿瘤药物,可以抑制DU145和PC-3癌细胞的生长、转移和糖酵解。这一发现可能为前列腺癌的临床治疗提供一种可能的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Brusatol inhibits the growth of prostate cancer cells and reduces HIF-1α/VEGF expression and glycolysis under hypoxia
Prostate cancer (PCa) has a high rate of morbidity and mortality, which urges us to find a unique and effective drug for treatment. Brusatol, a triterpenoid-degraded derivative, possesses antitumor activities. However, the significance of Brusatol in prostate cancer has not yet been completely elucidated. Therefore, this study aimed to explore how Brusatol affected prostate cancer cells. DU145 and PC-3 cell lines were chosen as experimental models. After Brusatol was added to relevant cells in culture, CCK-8 and colony formation experiments were used to assess cell viability; apoptosis rates were calculated using flow cytometry; and transwell assays were utilized to assess cell migration and invasion ability. Vimentin, N-cadherin, E-cadherin, zonula occludens-1 (ZO-1), hypoxia-inducible factor 1α (HIF-1α), vascular endothelial growth factor (VEGF), glucose transporter-1 (GLUT1), hexokinase 2 (HK2), and lactate dehydrogenase (LDHA) protein expression were evaluated by western blotting, and glucose consumption in cells was assessed using related equipment. In DU145 and PC-3 cells, Brusatol drastically reduced cell proliferation, promoted apoptosis, hindered migration and invasion. Considerably decreased HIF-1α and VEGF protein levels under hypoxia were detected. Furthermore, the expression of GLUT1, HK2, and LDHA was diminished, resulting in decreased glucose consumption in a Brusatol concentration-dependent manner. These findings demonstrate that Brusatol serves as a potent antitumor drug that suppresses DU145 and PC-3 cancer cell growth, metastasis, and glycolysis. This discovery could provide a possible clinical treatment strategy for prostate cancer.
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