基于RAPD标记的可可抗病材料遗传多样性分析

IF 1.3 4区农林科学 Q3 AGRONOMY

Crop Breeding and Applied Biotechnology Pub Date : 2004-03-30 DOI:10.12702/1984-7033.V04N01A03

F. Faleiro, U. Lopes, M. M. Yamada, G.R.P. Melo, W. Monteiro, J. L. Pires, J. B. Rocha, Rita C.S Bahia, L. M. C. Gomes, I. S. Araújo, A. S. G. Faleiro

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引用次数: 9

摘要

为了增加抗丛枝病的遗传多样性，对巴西巴伊亚州商业种植园的59棵可可树进行了选择和克隆。利用RAPD标记对这些无性系的多样性进行评价。将它们的系谱与在该州栽培的大多数杂交品种中用作亲本的传统抗性来源进行了比较。共获得RAPD条带106条，其中多态条带占85.8%。无性系间遗传距离为0.04 ~ 0.41。MDS图显示无性系间存在较大的遗传变异。对Scavina-6和Scavina-12这两个重要的抗病源进行了罕见的遗传标记分析，结果表明这两个源与大多数种质的亲缘关系密切。然而，一些加入的品种没有出现任何罕见的Scavina标记，这表明可能存在不同的等位基因，这可能有助于扩大对女巫扫帚的抗性育种计划的基因库。

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Genetic diversity of cacao accessions selected for resistance to witches' broom based on RAPD markers

To increase the genetic diversity sources of resistance to witches' broom disease, 59 cacao trees of commercial plantations of the State of Bahia, Brazil, were selected and cloned. The diversity of these clones was evaluated with RAPD markers. Their pedigree was investigated by comparison to traditional resistance sources used as parents for most hybrid mixtures cultivated in the State. One hundred and six RAPD bands were obtained, 85.8% of which were polymorphic. Genetic distances among the clones varied from 0.04 to 0.41. The MDS plot showed large genetic variability among clones. Analyses based on rare markers inherent to Scavina-6 and Scavina-12 clones, which represent important resistance sources, showed a close relationship of these sources to most accessions. However, some accessions did not present any of the rare Scavina markers, suggesting potentially different alleles, which could contribute to broaden the gene pool of breeding programs for resistance to witches' broom.

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来源期刊

Crop Breeding and Applied Biotechnology AGRONOMY-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.70

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.