SOX10可区分室管膜瘤中的毛细胞和毛粘液样星形细胞瘤,但在婴幼儿和大龄儿童室管膜瘤或不同分子亚群中的表达水平无差异

B. Kleinschmidt-DeMasters, A. Donson, A. Richmond, M. Pekmezci, T. Tihan, N. Foreman
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引用次数: 15

摘要

SOX10在非肿瘤性少突胶质细胞发育中很重要,但mRNA转录物和蛋白表达在更多种类的中枢神经系统胶质肿瘤中比在少突胶质细胞瘤中被发现。我们之前证明了毛细胞星形细胞瘤(PAs)中SOX10 mRNA和蛋白的高水平,但室管膜瘤(epn)中没有。我们现在将这些研究扩展到研究这两种影响婴儿的肿瘤亚群,毛液样星形细胞瘤(PMAs)和婴儿(<1岁)室管膜瘤(iEPNs)。通过基因表达芯片分析,我们发现iEPNs和所有年龄较大的儿童EPNs的SOX10表达水平非常低,平均比正常对照组织低7.1倍。EPN组与iEPN组SOX10表达差异无统计学意义。PAs/PMAs的转录水平分别比正常组织高24.1/29.4倍。通过对成人、儿童和婴儿EPNs以及PAs/ pma的免疫组织化学分析,我们发现来自多个解剖位置和两个年龄组的EPNs (n = 228)从未显示SOX10的3+弥漫性核免疫染色;大多数得分为0或1+。相反,几乎所有的儿童和成人PAs和pma (n = 47)都被评为3+。这些结果表明,在特定情况下,SOX10免疫组织化学可以补充PMA和PA的诊断,并有助于将它们与epn区分开来。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SOX10 Distinguishes Pilocytic and Pilomyxoid Astrocytomas From Ependymomas but Shows No Differences in Expression Level in Ependymomas From Infants Versus Older Children or Among Molecular Subgroups
SOX10 is important in nonneoplastic oligodendroglial development, but mRNA transcripts and protein expression are identified in a wider variety of CNS glial neoplasms than oligodendrogliomas. We previously demonstrated high levels of SOX10 mRNA and protein in pilocytic astrocytomas (PAs) but not ependymomas (EPNs). We now extend these studies to investigate subsets of these 2 tumors that affect infants, pilomyxoid astrocytomas (PMAs) and infant (<1 year) ependymomas (iEPNs). By gene expression microarray analysis, we found that iEPNs and all EPNs in older children showed very low SOX10 expression levels, on average 7.1-fold below normal control tissues. EPN groups showed no significant difference in SOX10 expression between iEPN and EPN. PAs/PMAs had 24.1/29.4-fold higher transcript levels, respectively, than those in normal tissues. Using immunohistochemical analysis of adult, pediatric, and infantile EPNs and of PAs/PMAs, we found that EPNs from multiple anatomical locations and both age groups (n = 228) never showed 3+ diffuse nuclear immunostaining for SOX10; the majority were scored at 0 or 1+. Conversely, almost all pediatric and adult PAs and PMAs (n = 47) were scored as 3+. These results suggest that in select settings, SOX10 immunohistochemistry can supplement the diagnosis of PMA and PA and aid in distinguishing them from EPNs.
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