利用棘青霉生产高耐热漆酶促进未经处理的麦麸脱木质素

F. M. Olajuyigbe, Ademola K. Oduwole, Cornelius O. Fatokun
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引用次数: 0

摘要

木质素赋予植物细胞壁刚性,对纤维素的水解提出了挑战,这使得从木质素纤维素生产生物燃料成为一个压倒性的问题。这促使人们不断寻找新的木质素分解酶,特别是用于木质纤维素脱木质素的漆酶,以改善生物质的糖化。本研究报道了青霉和木霉两种漆酶在未经处理的麦麸上的生产、理化性质和脱木质素效率。以不同的农渣(麦麸、椰子壳和棕榈仁壳)为底物进行深层发酵,制备真菌漆酶。确定了生产漆酶的最佳底物。以纯漆酶为对照,在未经处理的麦麸上测定了粗酶的理化性质和漆酶的脱木质素效率。麦麸支持真菌最大限度地生产漆酶。漆酶产率最高,为22.5 U/mL。漆酶在pH 7.0和50℃条件下具有最佳活性,在90℃条件下培养2 h后,漆酶的残余活性为61.6%。在Cu2+的存在下,热稳定酶的活性增强。无细胞提取液对麦麸的生物脱木质素效率分别为95%、81.5%和63.5%。令人惊讶的是,商业漆酶的脱木质素效率要低得多,为33.42%。棘棘木漆酶具有较高的热稳定性和显著的脱木质素效率,是对未经处理的木质纤维素进行生物脱木质素处理的良好生物资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Production of Highly Thermostable Laccase from Penicillium spinulosum for Enhanced Delignification of Untreated Wheat Bran
Lignin confers rigidity on plant cell wall and poses a challenge to hydrolysis of cellulose, which makes production of biofuels from lignocellulose an overwhelming problem. This prompts continuous search for novel ligninolytic enzymes, especially, laccases for delignification of lignocellulose for improved saccharification of biomass. This study reports production, physicochemical properties and delignification efficiency of laccases from Penicillium and Trichoderma species on untreated wheat bran. Fungal laccases were produced using different agroresidues (wheat bran, coconut shell and palm kernel shell) as substrates in submerged fermentation. Best substrate for laccase production was determined. Physicochemical properties of crude enzymes and delignification efficiency of the laccases were determined on untreated wheat bran using pure laccase as control. Wheat bran supported maximum laccase production from fungi under study. Highest laccase yield of 22.5 U/mL was obtained from P. spinulosum. Laccase from P. spinulosum was optimally active at pH 7.0 and 50 °C and exhibited remarkable high thermostability with 61.6% residual activity at 90 °C after 2 h incubation. The activity of the thermostable enzyme was enhanced in the presence of Cu2+. Biodelignification efficiency of cell-free extract from P. spinulosum, T. koningii, and P. restrictum on wheat bran were 95%, 81.5% and 63.5%, respectively. Surprisingly, a much lower delignification efficiency of 33.42% was obtained with commercial laccase from Trametes versicolor. The high thermostability and striking delignification efficiency of laccase from P. spinulosum make the enzyme a good bioresource for biodelignification of untreated lignocellulose for biofuel production.
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