采用迪安流分馏的微流体混合和颗粒聚焦系统

IF 1.5 4区 材料科学 Q4 MATERIALS SCIENCE, MULTIDISCIPLINARY
Alexander Wiede, O. Stranik, A. Tannert, U. Neugebauer
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引用次数: 0

摘要

增材制造工艺领域的最新发展带来了巨大的技术进步,为微流体领域开辟了方向。例如,用于3D打印的新型柔性材料允许在微流体原型开发中替代聚二甲基硅氧烷(PDMS)。三维打印微流体组件开辟了新的领域,特别是对生物细胞(如真核细胞或细菌)的自动处理。在这里,我们展示了如何使用3D打印概念实现生物细胞的被动混合和被动分离过程。这种技术有助于低成本的实验设置,易于修改和采用特定的检测和诊断目的。特别是基于熔融沉积建模和立体光刻的印刷技术,并讨论了它们的实现。增材技术使倍增混合器的制造成为可能,它克服了当前柱式或曲线式技术的缺点,使生物细胞在不影响生存能力的情况下也能有效混合。利用标准的微流体元件和最先进的3D打印技术,我们实现了一个基于Dean流破碎的分离系统,而不使用PDMS。特别地,我们描述了使用3d打印螺旋缠绕颗粒流的毛细管和用于出口颗粒分离的新芯片设计。我们通过从含有大颗粒(~12 μ m,真核细胞的典型尺寸)和小颗粒(~2 μ m,细菌或小酵母的典型尺寸)的颗粒混合物中成功分离出~12 μ m大小的颗粒,证明了该系统的功能。利用这种装置分离真核细胞和细菌,我们可以证明细胞活力不受通过微流体系统的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microfluidic System for Cell Mixing and Particle Focusing Using Dean Flow Fractionation
Recent developments in the field of additive manufacturing processes have led to tremendous technological progress and opened directions for the field of microfluidics. For instance, new flexible materials for 3D printing allow the substitution of polydimethylsiloxane (PDMS) in microfluidic prototype development. Three-dimensional-printed microfluidic components open new horizons, in particular for the automated handling of biological cells (e.g., eukaryotic cells or bacteria). Here, we demonstrate how passive mixing and passive separation processes of biological cells can be realized using 3D printing concepts for rapid prototyping. This technique facilitates low-cost experimental setups that are easy to modify and adopt for specific detection and diagnostic purposes. In particular, printing technologies based on fused deposition modeling and stereolithography are used and their realization is discussed. Additive technologies enable the fabrication of multiplication mixers, which overcome shortcomings of current pillar or curve-based techniques and enable efficient mixing, also of biological cells without affecting viability. Using standard microfluidic components and state-of-the art 3D printing technologies, we realize a separation system based on Dean flow fragmentation without the use of PDMS. In particular, we describe the use of a 3D-printed helix for winding a capillary for particle flow and a new chip design for particle separation at the outlet. We demonstrate the functionality of the system by successful isolation of ~12 µm-sized particles from a particle mixture containing large (~12 µm, typical size of eukaryotic cells) and small (~2 µm, typical size of bacteria or small yeasts) particles. Using this setup to separate eukaryotic cells from bacteria, we could prove that cell viability is not affected by passage through the microfluidic systems.
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来源期刊
Micro & Nano Letters
Micro & Nano Letters 工程技术-材料科学:综合
CiteScore
3.30
自引率
0.00%
发文量
58
审稿时长
2.8 months
期刊介绍: Micro & Nano Letters offers express online publication of short research papers containing the latest advances in miniature and ultraminiature structures and systems. With an average of six weeks to decision, and publication online in advance of each issue, Micro & Nano Letters offers a rapid route for the international dissemination of high quality research findings from both the micro and nano communities. Scope Micro & Nano Letters offers express online publication of short research papers containing the latest advances in micro and nano-scale science, engineering and technology, with at least one dimension ranging from micrometers to nanometers. Micro & Nano Letters offers readers high-quality original research from both the micro and nano communities, and the materials and devices communities. Bridging this gap between materials science and micro and nano-scale devices, Micro & Nano Letters addresses issues in the disciplines of engineering, physical, chemical, and biological science. It places particular emphasis on cross-disciplinary activities and applications. Typical topics include: Micro and nanostructures for the device communities MEMS and NEMS Modelling, simulation and realisation of micro and nanoscale structures, devices and systems, with comparisons to experimental data Synthesis and processing Micro and nano-photonics Molecular machines, circuits and self-assembly Organic and inorganic micro and nanostructures Micro and nano-fluidics
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