微卫星标记在大豆包囊线虫抗性辅助选择中的应用

IF 1.3 4区农林科学 Q3 AGRONOMY

Crop Breeding and Applied Biotechnology Pub Date : 2004-03-30 DOI:10.12702/1984-7033.V04N01A05

A. O. Mauro, S. H. Unêda-Trevisoli, S. Mauro, M. Costa, R. C. Oliveira, N. E. Arantes

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引用次数: 5

摘要

对4个微卫星序列在大豆品种DNA和分离基因型中进行了检测。其中3个与分子连锁组G上的抗性位点rhg1相近(sat309、Sat_168、Sat_163)， 1个与分子连锁组A2上的抗性位点Rhg4相近(Sat_162)。以抗性品种Lideranca和Renasscenca以及易感品种Cristalina和ocear -4为对照，对先前被分类为囊线虫(SCN)抗性和其他未知反应的后代进行了检测。抗SCN的最佳引物是Sat_162。用Sat_162检测的20个分离子代，纯合子抗性基因型的条带为150 bp，易感基因型的条带为200 bp，杂子型的条带均为150 bp。已有研究表明，该微卫星标记可有效筛选携带北京衍生抗性的基因型。“北京”参与了本研究中评估的所有分离后代的谱系。结果表明，Sat_162区分了大豆3号小种对SCN的抗性和易感基因型。

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Efficiency of microsatellite markers in assisted selection for resistance to soybean cyst nematode (race 3)

Four microsatellite sequences were tested in soybean DNA from cultivars and segregating genotypes. Three of them were close to the resistance locus rhg1 on molecular linkage group G (Satt309, Sat_168, Sat_163) and one was close to Rhg4 locus on group A2 (Sat_162). Progenies previously classified as cyst nematode (SCN) resistant and others with unknown reaction were tested, using the resistant cultivars Lideranca, and Renasscenca and the susceptibles 'Cristalina' and 'OCEPAR-4' as control. The best primer for resistance to SCN was Sat_162. Twenty segregant progenies tested with Sat_162 presented a 150 bp band for homozygous resistant genotypes and 200 bp for susceptible ones, and both for the heterozigous genotypes. Previous studies have shown that this microsatellite marker is efficient to select genotypes carrying Peking-derivative resista nce. 'Peking' takes part in the in the genealogy of all segregant progenies evaluated in this study. Thus, the obtained results showed that Sat_162 distinguished resistant and susceptible soybean genotypes to SCN, race 3.

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来源期刊

Crop Breeding and Applied Biotechnology AGRONOMY-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.70

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.