Saba Khan, Mohd Anas Shaikh, Nehal Shaikh, J. Agnihotri
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引用次数: 0
摘要
建立了一种简单、快速、准确、经济、精密的紫外/可见分光光度法。常用溶剂的选择是必要的，因此分析了甲醇、乙醇、乙腈和0.1 N HCL的不同溶剂范围和各种缓冲液的不同浓度范围。因此选择0.1 N HCL作为溶剂。咖啡因在273nm处吸光度最大。药物中咖啡因在3 ~ 18 μg/mL的浓度范围内符合Beer - Lambert定律。咖啡因的检出限为0.5476 μg/mL，检出限为1.6594 μg/mL。从线性度、精密度、精密度、LOD、LOQ、回收率等方面对该方法进行了定量评价。该方法简便、简便，适用于原料药中咖啡因的分析。
Analytical method development and validation of UV-visible spectrophotometric method for the estimation of CNS stimulant drug in bulk
A simple, rapid, accurate, economical and precise UV/VIS method has been developed and validated. Choices of a common solvent were essential so various solvent ranges including methanol, ethanol, acetonitrile, and 0.1 N HCL, and various concentrations ranges of various buffers were analyzed. Hence 0.1 N HCL was selected as a solvent for the proposed method. Caffeine showed maximum absorbance at 273nm.Drug obey Beer Lambert’s law in the concentration range of 3-18 μg/mL for Caffeine. The LOD and LOQ were found to be 0.5476 μg/mL and 1.6594 μg/mL for Caffeine. The method was quantitatively evaluated in terms of linearity, precision, precision, LOD, LOQ and recovery. The method is simple, convenient and suitable for the analysis of Caffeine in bulk drugs.
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