优化了丝状真菌中高效融合PCR产生敲除载体的引物及其他关键条件。

Fungal Genetics Reports Pub Date : 2013-12-01 DOI:10.4148/1941-4765.1006

Nicholas Harrison, Brad Cavinder, J. Townsend, F. Trail

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引用次数: 3

摘要

简化大量基因功能研究的方法对于充分利用真菌现有的重要基因组资源至关重要。融合PCR通常用于将DNA片段连接在一起，特别是在真菌中用于基因替换的DNA片段的构建中。在这里，我们提出了高效的引物，可靠地指导融合和扩增，以产生基因敲除的结构。

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Optimized primers and other critical conditions for efficient fusion PCR to generate knockout vectors in filamentous fungi.

Methods to streamline functional studies of large numbers of genes are essential to fully utilize the significant genomic resources now available for fungi. Fusion PCR is often used to join pieces of DNA together, particularly in the construction of DNA fragments for gene replacement in fungi. Here we present high-efficiency primers which reliably direct fusion and amplification to generate constructs for gene knockouts.

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Fungal Genetics Reports

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