用[15N]同位素稀释技术估计仔猪内源性氮损失:在小麦和黑麦基础日粮中添加木聚糖酶的影响

S. Dänicke, H. Kluge, G. Dusel, H. Jeroch
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引用次数: 3

摘要

试验开始时体重为19.4±1.4 kg的仔猪,在7 d内口服([15N]H4SO4(比饲粮氮高出1.26 [l5N] -原子百分比)氮池进行标记。标记期后为7 d的平衡期,不饲喂标记化合物。两种试验饲粮分别以小麦(53%)和黑麦(25%)为基础,在两个试验期间分别饲喂或不饲喂含木聚糖酶的酶制剂。此外,在试验的第二阶段,饲粮中添加了一种不消化标记物,以便计算猪消化道后续部分的内源性N -损失。这些内源性氮损失在实验结束时通过分析粪便、摄食和尿液来估计[15N] -富集,假设尿液的[15N] -富集代表前体池的[15N] -富集。添加木聚糖酶对任何测量部位(胃、小肠3段、全消化道)的内源性氮损失均无显著影响。6头猪小肠末段和整个消化道的内源氮占总氮的平均比例分别为42±11%和56±5%,对应的内源氮损失分别为2.8±1.3 g N/kg DM和2.0±0.3 g N/kg DM。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Endogenous N‐losses in piglets estimated by a [15N]‐isotope dilution technique: Effect of xylanase addition to a wheat and rye based diet
The nitrogen pool of piglets weighing 19.4 ± 1.4 kg at the beginning of the experiment was labeled with an oral application of ([15N]H4SO4 (1.26 [l5N]‐atom percent excess of dietary N) over a period of 7 d. The labeling period was followed by an equilibration period of 7 d without feeding the labeling compound. The two experimental diets were based on wheat (53%) and rye (25%) and were fed either with or without a xylanase containing enzyme preparation over both experimental periods. Additionally, diets were supplemented with an indigestible marker during the 2nd period of the experiment to allow the calculation of endogenous N‐Iosses in subsequent segments of the digestive tract of the pigs. These endogenous N‐losses were estimated at the end of the experiment by analyzing feces, ingesta and urine for [15N]‐enrichment assuming that [l5N]‐enrichment of urine represents the [15N]‐enrichment of the precursor pool. Endogenous N‐losses were not significantly affected by xylanase addition at any measurement site (stomach, 3 sections of the small intestine, total digestive tract). Endogenous N‐proportions of total nitrogen amounted on average for the six pigs to 42 ± 11% and 56 ± 5% at the last section of the small intestine and over the whole digestive tract, respectively, which corresponded to endogenous N‐losses of 2.8 ± 1.3 g N/kg DM and 2.0 ± 0.3 g N/kg DM, respectively.
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