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引用次数: 1934
摘要
用紫外可见光谱法定量测定绿色植物组织全色素提取物中的叶绿素a、叶绿素b和类胡萝卜素，由于样品、溶剂系统和分光光度计的选择而变得复杂。不同的植物色素在重叠的光谱区域吸收光，这取决于所选择的系统。本单元讨论了用于解释这种重叠的方法，通过应用方程来精确定量测定叶子或水果中相同色素提取物中的Chl a, Chl b和总类胡萝卜素。关于绿色植物组织全色素提取物中Chl a和Chl b的光谱特征、比吸收系数和定量测定的一般信息可在Sestak(1971)和Lichtenthaler(1987)中找到。有关Chl结构，请参见单元F4.1。
Chlorophylls and Carotenoids: Measurement and Characterization by UV‐VIS Spectroscopy
The quantitative determination of chlorophyll (Chl) a, Chl b, and carotenoids in a wholepigment extract of green plant tissue by UVVIS spectroscopy is complicated by the choice of sample, solvent system, and spectrophotometer used. The various plant pigments absorb light in overlapping spectral regions, depending on the system selected. This unit discusses methods used to account for such overlap by applying equations for accurate quantitative determination of Chl a, Chl b, and total carotenoids in the same pigment extract of leaves or fruits. General information on the spectroscopic characteristics of Chl a and Chl b, their specific absorption coefficients, and their quantitative determination in a whole-pigment extract of green plant tissues can be found in Sestak (1971) and Lichtenthaler (1987). For Chl structures, see UNIT F4.1.
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