马铃薯普通痂病对叶片生化反应的影响

Q4 Agricultural and Biological Sciences

International Journal of Phytopathology Pub Date : 2021-12-30 DOI:10.33687/phytopath.011.03.4445

Kamra Mehmood, S. T. Sahi, M. Atiq, M. Shahid

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引用次数: 0

摘要

本研究于2017 - 2020年在巴基斯坦费萨拉巴德Ayub农业研究所植物病理学研究所的研究区域设计。在播种后35天、65天和95天分别采集接种和未接种马铃薯高感(FD 76-36)、中感(FD 73-110)和抗性(Esmee) /试验品系的鲜叶，进行生化分析。测定其超氧化物歧化酶活性、过氧化氢酶活性、过氧化酶活性、蛋白质和总酚含量。接种35、65和95 d后，FD 76-36的SOD活性最低(67.733、45.637、24.910)%，其次是FD 73-110(70.303、57.893、42.513)%和Esmee(84.567、65.167、45.873)%。接种35、65、95 d后，FD 76-36的CAT活性最低，分别为10.990、7.473、3.413 %，Esmee和FD 73-110分别为13.537、9.630、8.147 %和19.277、8.147、6.170 %。接种35、65和95 d后，FD 76-36的POD活性最低(0.2133、0.2767、0.3600)%，其次是FD 73-110(0.2900、0.5567、0.6300)%和Esmee(0.8800、1.2733、1.5433)%。接种35、65和95 d后，FD 76-36蛋白含量最低(3.097、2.873、1.260)%，FD 73-110蛋白含量最低(7.907、5.423、4.267)%，Esmee蛋白含量最低(12.163、8.633、5.127)%。接种35、65和95 d后，FD 76-36的TPC表达量最低(136.31、115.58、70.77)%，其次是FD 73-110(165.85、136.75、86.89)%和Esmee(188.42、158.38、109.00)%。试验采用随机完全区组设计(RCBD)， 3个重复。实验数据采用Fisher方差分析技术进行分析，处理手段采用5%概率水平的最小显著性差异(LSD)检验。

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Alteration in Biochemical Responses in Leaves of Potato due to Common Scab Disease

Current Study was designed at the research area of Plant Pathology Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan during the 2017– 2020. Fresh leaves of inoculated and un-inoculated potato varieties highly susceptible (FD 76-36), moderately susceptible (FD 73-110), and resistant (Esmee) / test lines were collected during 2017-18 and 2018-19, at 35, 65 and 95 days after sowing for biochemical analysis. Superoxide dismutase activity, Catalase activity, Per-oxidase activity, Protein and Total phenolics contents were quantified. FD 76-36 exhibited minimum SOD activity (67.733, 45.637, 24.910) %, followed by FD 73-110 (70.303,57.893, 42.513) % and Esmee (84.567, 65.167, 45.873) % after 35, 65 and 95 days of inoculation respectively as compared to control . FD 76-36 expressed minimum CAT activity (10.990, 7.473, 3.413) %, followed by Esmee (13.537, 9.630, 8.147) % and FD 73-110 (19.277, 8.147, 6.170) % after 35, 65 and 95 days of inoculation respectively as compared to control . FD 76-36 exhibited minimum POD activity (0.2133, 0.2767, 0.3600) %, followed by FD 73-110 (0.2900, 0.5567 , 0.6300)% and Esmee (0.8800, 1.2733 , 1.5433)% after 35, 65 and 95 days of inoculation respectively as compared to control. FD 76-36 expressed minimum protein contents (3.097, 2.873, 1.260) %, followed by FD 73-110 (7.907, 5.423, 4.267) % and Esmee (12.163, 8.633, 5.127) % after 35, 65 and 95 days of inoculation respectively as compared to control. FD 76-36 expressed minimum TPC (136.31, 115.58, 70.77) %, followed by FD 73-110 (165.85, 136.75, 86.89) % and Esmee (188.42, 158.38, 109.00) % after 35, 65 and 95 days of inoculation respectively as compared to control. Experiment was conducted with a randomized complete block design (RCBD) and three replications. The experimental data was analyzed using Fisher’s analysis of variance technique and treatment means were compared by least significance difference (LSD) test at 5% probability level.

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来源期刊

International Journal of Phytopathology Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

1.20

自引率

0.00%

发文量