{"title":"川芎嗪通过调节miR-211/USP47通路增强瑞芬太尼对心肌缺血再灌注损伤的保护作用","authors":"Xin Li, D. Xia","doi":"10.15586/qas.v14i4.1179","DOIUrl":null,"url":null,"abstract":"Purpose: To assess the effects of ligustrazine on the progression of hypoxia-reoxygenation (H/R), and explore the possible role of miR-211 in myocardial ischemia-reperfusion (I/R) injury and identify its potential targeting gene. Methods: The level of miR-211 in H/R-induced cells was detected by quantitative polymerase chain reaction. CCK-8, flow cytometry (FCM), and Western blot assays were performed to examine the effect of miR-211 and USP47 on the role of remifentanil against H/R injury. Bioinformatic analysis and luciferase and Western blot assays were performed to identify and verify the potential target of miR-211. CCK-8 and FCM assays were performed to detect the mechanism of ligustrazine and miR-211 in the protection of remifentanil against H/R-induced cells. Results: Ligustrazine enhanced the effect of remifentanil on H/R-induced cardiomyocytes. MiR-211 enhanced the protective effect of remifentanil against H/R injury. Down-regulation of USP47 enhanced the protective effect of remifentanil against H/R injury. Ligustrazine enhanced the protective effect of remifentanil against H/R injury through miR-211/USP47 pathway. Conclusion: Ligustrazine enhanced the protective effect of remifentanil on myocardial I/R injury by regulating miR-211/USP47 pathway.","PeriodicalId":20738,"journal":{"name":"Quality Assurance and Safety of Crops & Foods","volume":"16 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ligustrazine enhances the protective effect of remifentanil on myocardial ischemia-reperfusion injury by regulating miR-211/USP47 pathway\",\"authors\":\"Xin Li, D. Xia\",\"doi\":\"10.15586/qas.v14i4.1179\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Purpose: To assess the effects of ligustrazine on the progression of hypoxia-reoxygenation (H/R), and explore the possible role of miR-211 in myocardial ischemia-reperfusion (I/R) injury and identify its potential targeting gene. Methods: The level of miR-211 in H/R-induced cells was detected by quantitative polymerase chain reaction. CCK-8, flow cytometry (FCM), and Western blot assays were performed to examine the effect of miR-211 and USP47 on the role of remifentanil against H/R injury. Bioinformatic analysis and luciferase and Western blot assays were performed to identify and verify the potential target of miR-211. CCK-8 and FCM assays were performed to detect the mechanism of ligustrazine and miR-211 in the protection of remifentanil against H/R-induced cells. Results: Ligustrazine enhanced the effect of remifentanil on H/R-induced cardiomyocytes. MiR-211 enhanced the protective effect of remifentanil against H/R injury. Down-regulation of USP47 enhanced the protective effect of remifentanil against H/R injury. Ligustrazine enhanced the protective effect of remifentanil against H/R injury through miR-211/USP47 pathway. Conclusion: Ligustrazine enhanced the protective effect of remifentanil on myocardial I/R injury by regulating miR-211/USP47 pathway.\",\"PeriodicalId\":20738,\"journal\":{\"name\":\"Quality Assurance and Safety of Crops & Foods\",\"volume\":\"16 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-10-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Quality Assurance and Safety of Crops & Foods\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15586/qas.v14i4.1179\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Quality Assurance and Safety of Crops & Foods","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15586/qas.v14i4.1179","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Ligustrazine enhances the protective effect of remifentanil on myocardial ischemia-reperfusion injury by regulating miR-211/USP47 pathway
Purpose: To assess the effects of ligustrazine on the progression of hypoxia-reoxygenation (H/R), and explore the possible role of miR-211 in myocardial ischemia-reperfusion (I/R) injury and identify its potential targeting gene. Methods: The level of miR-211 in H/R-induced cells was detected by quantitative polymerase chain reaction. CCK-8, flow cytometry (FCM), and Western blot assays were performed to examine the effect of miR-211 and USP47 on the role of remifentanil against H/R injury. Bioinformatic analysis and luciferase and Western blot assays were performed to identify and verify the potential target of miR-211. CCK-8 and FCM assays were performed to detect the mechanism of ligustrazine and miR-211 in the protection of remifentanil against H/R-induced cells. Results: Ligustrazine enhanced the effect of remifentanil on H/R-induced cardiomyocytes. MiR-211 enhanced the protective effect of remifentanil against H/R injury. Down-regulation of USP47 enhanced the protective effect of remifentanil against H/R injury. Ligustrazine enhanced the protective effect of remifentanil against H/R injury through miR-211/USP47 pathway. Conclusion: Ligustrazine enhanced the protective effect of remifentanil on myocardial I/R injury by regulating miR-211/USP47 pathway.
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