生物电系统持续直接电刺激对实验性单面神经损伤下失神经圆肌组织影响的形态学评价

Q4 Medicine

Otorhinolaryngology Clinics Pub Date : 2022-05-11 DOI:10.37219/2528-8253-2022-1-11

A. Bobrov, Oleg M. Borisenko, Anastasia E. Pedachenko, T. Malysheva, Alexander V. Papp

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引用次数: 0

摘要

在实验性面神经麻痹(FNP)中，使用直接电刺激使去神经圆眼缩短，使眼缝完全闭合。本研究的目的是研究长时间双相脉冲直接电刺激下神经形态的变化。材料与方法:以12只成年动物为实验对象，建立单侧面神经损伤并发眼轮匝肌麻痹模型。实验动物分为两组:对照组和对照组。主组植入闭眼生物电装置的刺激部分，测试其实时工作模式下长时间刺激眼轮匝肌的能力。对照组对眼轮匝肌无明显影响。30 d后，所有动物均行面神经麻痹侧眼轮匝肌消除术，并进行形态学检查。结果:采用非参数U-Mann-Whitney标准对Ki-67增殖指数变化进行组间比较分析，结果显示，实验组与对照组相比，电刺激组的Ki-67增殖指数受电刺激的影响随参数的增加而增加，从对照组的28,3 (25,0;30.8)至30.9 (28.8;33.3)或1.1倍(p=0.04);线粒体光电流密度的变化分析显示，对照组和实验组之间存在统计学差异，电刺激组的该参数从10.5 (6.3;14.4)到15.8(12.8 17,6)，即1.5倍(p=0.0004);与对照组相比，电刺激组的参数从48,9 (40,6;63.0)至64.4 (60.0;72.4)，即以1.3为因子(p = 0.00005)。结论:电刺激对神经成像后肌肉骨骼器官的影响评估(实验中)显示了结构和超微结构的变化，表明在直接电刺激的影响下，研究中肌纤维再生的形态功能活性增加。此外，直接电刺激30天未见特异性病理改变。

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Morphological evaluation of the effect of continuous direct electrostimulation with the help of the bioelectric system on the denervated circular musculature of the eye in case of experimental single facial nerve injury

The use of direct electrical stimulation to produce shortening of the denervated circular oculus in experimental facial nerve palsy (FNP) allows for complete closure of the ocular slit. The aim of this study is to study morphological changes in the nerves under the influence of direct electrical stimulation with biphasic pulses over a long period of time. Materials and methods: A model of a single facial nerve injury with consequent paralysis of orbicularis oculi muscle was created in the experiment on 12 adult animals. The animals were divided into 2 groups: main and control. In the main group, the stimulating part of the bioelectric device for eye closure was implanted, after which the ability to work in real-time mode to perform long-duration stimulation of orbicularis oculi muscle was tested. The control group did not receive any effect effects on orbicularis oculi muscle. After 30 days, all the animals underwent orbicularis oculi muscle elimination on the side of the facial nerve palsy with the following morphological examination. Results: An intergroup comparative analysis using the nonparametric U-Mann-Whitney criterion for changes in the Ki-67 proliferation index revealed a statistically significant difference between the comparison group and the experimental group, that was affected by the electrical stimulation with the increase of the parameter for the group with electrical stimulation according to the control group from 28,3 (25,0; 30.8) to 30.9 (28.8; 33.3), or 1.1 times (p=0.04); Analysis of variation in mitochondrial photocurrent density revealed statistically significant differences between the control group and the experimental group, with the increase of this parameter for the group with electrical stimulation compared with the control group from 10.5 (6.3; 14.4) to 15.8 (12.8 17,6), i.e. by 1.5 times (p=0.0004); a statistically significant increase of the parameter for the group with electrical stimulation versus the control group from 48,9 (40,6; 63.0) to 64.4 (60.0; 72.4), i.e. by a factor of 1.3 (p = 0.00005). Conclusions: The assessment of the effects of electrostimulation on the musculoskeletal apparatus after neuroraphy (in the experiment) revealed structural and ultrastructural changes, indicating the increased morphofunctional activity of renewed regeneration of muscle fibers in the study under the influence of direct electrical stimulation. In addition, no specific pathological changes were detected under the influence of direct electrostimulation for 30 days.

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来源期刊

Otorhinolaryngology Clinics Medicine-Otorhinolaryngology

CiteScore

0.10

自引率

0.00%

发文量

期刊介绍： Otorhinolaryngology Clinics: An International Journal is an International periodical devoted at exploring connections between clinical experience and world literature, and understanding of various pathologies and diseases related to the ear, nose and throat. Issues of recent advancements and research related to disease, illness, health and medical science are examined through various evidence-based clinical research studies. This journal proposes to serve as a collection of clinical notes, with an international perspective, along with the recent advances for postgraduates and consultants. The readership for this journal would include a wide variety of healthcare professionals, such as otolaryngologists, head and neck surgeons, ENT nurses as well as scholars and academicians in the field of medicine, trauma, surgery, etc. This journal aims to encourage the analysis of clinical data from various centers all over the world using standardized protocols to develop an international consensual perspective on the management of disorders related to the field of otorhinolaryngology. Recently, we have introduced "Case Reports", "How I Do It" and "Original Research" categories in the process of expanding the scope of the journal. Thisis a peer-reviewed journal of which three issues would be published each year. Each future issue will cover a different topic of special interest in the field of otorhinolaryngology and head and neck surgery. This issue is the first of its kind dedicated to "anesthesia in otorhinolaryngology" and contains a compilation of articles by experienced anesthesiologists dealing with a large volume of ENT and related surgeries. In each issue, the editors give their perspective based on the submitted articles. All non invited articles are peer-reviewed. Peer-revieweing helps in providing unbiased, independent, critical assessment of the results of the research study in question including the scientific process.