皮皮草乙醇提取物和乙酸乙酯部位的急性毒性及降糖活性评价

IF 0.7 4区医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas Pub Date : 2023-09-30 DOI:10.37360/blacpma.23.22.5.44

Jymmys Lopes dos Santos, A. M. O. E. Silva, Lúcio Marques Vieira Souza, Raquel Oliveira Pereira, C. A. Lima, L. Abreu, M. Montalvao, C. B. Correa, T. K. Rabelo, M. C. Duarte, F. Aidar, J. Tavares, C. Estevam, A. C. Marçal

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引用次数: 11

摘要

为了进一步了解其抗糖尿病潜能和毒性，本研究旨在对香豆嫩枝乙醇提取物和乙酸乙酯部位的急性毒性和抗糖尿病活性进行评价。对其化学成分和急性毒性进行了研究。对四氧嘧啶诱导的糖尿病大鼠进行100 mg/kg (p.o)剂量的提取液和馏分试验，测定其体重增加、血清和组织中葡萄糖、血脂和氧化应激指标。体外抗氧化活性测定。鉴定了獐牙菜苦苷、龙胆苦苷、脱氧酸、clovin和robinin及其对香豆香酯。提取物和馏分被列为安全(5类)。在糖尿病大鼠中，苦参能降低血糖，并伴有体重恢复增加和氧化应激标志物的减弱。对1,1-二苯基-2-吡啶肼基(DPPH)和2,2'-氮基-双(3-乙基苯并噻唑-6-磺酸)自由基(ABTS)自由基的清除能力和还原能力均高于提取物。苦参提取物及部位对糖尿病无明显毒性，可作为治疗糖尿病的替代药物进行研究。

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Evaluation of the acute toxicity and antidiabetic activity of Coutoubea spicata “nicolau” ethanolic extract and ethyl acetate fraction

In order to understand antidiabetic potential and toxicity, this study aimed to evaluate the acute toxicity and antidiabetic activity of ethanolic extract and ethyl acetate fraction obtained from Coutoubea spicata “nicolau” shoots. Chemical constituents and acute toxicity were investigated. In alloxan-induced diabetic rats, extract and fraction were tested at dose of 100 mg/kg, p.o. Body weight gain, glucose, lipid profile and oxidative stress markers in serum and tissues were determined. In vitroantioxidant activity was performed. Swertiamarin, gentiopicrin, deoxyloganic acid, clovin and robinin, and their p-coumaric ester were identified. Extract and fraction were classified as safe (category 5). In diabetic rats, Coutoubea spicata reduced glycaemia, which was accompanied by body weight recovery gain and attenuation in oxidative stress markers. Fraction showed scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical (ABTS) radicals and reducing power higher than that of the extract. Extract and fraction of Coutoubea spicata didn’t present significant toxicity and it can be investigated as a therapeutic alternative in diabetes.

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来源期刊

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas PHARMACOLOGY & PHARMACY-

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas (BLACPMA), [Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants]; currently edited by the publishing house MS-Editions, is a bi-monthly international publication that publishes original peerreviewed research in the field of medicinal and aromatic plants, with nearly 20 years of experience. BLACPMA is a scientific journal that publishes two types of articles: Reviews (only in English) and Original Articles (Spanish or English), its main lines of action being agronomy, anthropology and ethnobotany, industrial applications, botany, quality and standardization, ecology and biodiversity, pharmacology, phytochemistry, pharmacognosy, regulatory and legislative aspects. While all areas of medicinal plants are welcome and the experimental approaches used can be broad and interdisciplinary; other areas of research that are not mentioned depend on the Editorial Committee for their acceptance.