酞菲罗啉试剂分光光度法测定药物制剂中乙酰半胱氨酸

S. Hasan, S. Sultan
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引用次数: 2

摘要

提出了一种灵敏、快速的测定其制剂中乙酰半胱氨酸的分光光度法。该方法利用铁(III)在水介质中氧化所研究的药物化合物,生成的铁(II)与邻苯二酚试剂反应形成红色配合物,该配合物稳定且可溶于水,在534 nm处具有最大吸收。在5的浓度范围内,遵守啤酒定律。4 ~ 10.0µg。Ml -1的乙酰半胱氨酸。表观摩尔吸光度值为2.35×10 4 l.mol -1 .cm -1, Sandall灵敏度指数为0.00694µg。Cm -2,相对误差为-4.72 ~ 4.32%,相对标准偏差为0.627 ~ 3.54%,取决于浓度水平。该方法成功地测定了其药用制剂(囊(粉)、胶囊和注射剂中乙酰半胱氨酸的含量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Using of Bathophenanthroline Reagent for Spectrophotometric Estimation of Acetylcysteine in its Pharmaceutical Preparations
A sensitive and rapid spectrophotometric method was suggested for the estimation of acetylcysteine in its pharmaceutical formulations. The proposed method was depended on the oxidation of the studied drug compound by iron (III) in aqueous medium, and then reaction of produced iron (II) with bathophenanthroline reagent to form a red color complex, which is stable and soluble in water and exhibits a maximum absorption at 534 nm. Beer s law is obeyed over the concentration range of 5..4 to 10.0 µg.ml -1 of acetylcysteine. The apparent molar absorptivity value is 2.35×10 4 l.mol -1 .cm -1 and Sandall ’ s sensitivity index of 0.00694 µg.cm -2 , a relative error of -4.72 to 4.32% and a relative standard deviation of 0.627 to 3.54% depending on the concentration level. The proposed method was applied successfully to the determination of acetylcysteine in its pharmaceutical preparations as sachet (powder), capsule and injection.
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