不同温度下育比昆宁木薯基因型离体苗的生长和脯氨酸含量

Supatmi Supatmi, N. Rahman, N. Hartati
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引用次数: 0

摘要

木薯在逆境条件下具有不同的耐受机制,是气候变化条件下保障粮食安全的重要作物。然而,木薯在田间的可持续生长取决于许多因素,尤其是温度。本试验旨在研究10 Gy辐照后的乌比库宁在无激素固体培养基中25℃、28℃和30℃3种不同温度下培养4周后的生长性能和脯氨酸含量。每个处理为3个无性系外植体,5个重复。结果表明,在不同温度下,辐照苗的株高和叶片数均优于未辐照苗。在30℃下生长的幼苗生长性能最好。在25℃和30℃条件下,辐照后的乌壁昆宁植株脯氨酸含量较高,具有耐低温和耐高温的可能性。本研究对了解辐照后乌壁库宁对低温和高温的生长能力有初步的指导意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Growth and Proline Content of Irradiated in Vitro Shoots of Ubi Kuning Cassava Genotype Cultured at Different Temperatures
Cassava (Manihot esculenta) is an important crop to food security under climate change due to its various tolerance mechanism under stress conditions. However, the sustainable growth of cassava in the field depends on many factors especially temperature. The objective of the research was to investigate the growth performances and proline contents of irradiated Ubi Kuning at dosage of 10 Gy, cultured in Murashige Skoog (MS) hormone-free solid medium for 4 weeks at three different temperature treatments i.e 25oC, 28oC and 30oC. Each treatment consisted of 3 clone explants with 5 replicates. Results show that growth performances of irradiated plantlets were better compared to that of non-irradiated plantlets in terms of plant height and number of leaves at all temperature tested. The best growth performances were obtained from irradiated plantlets grown under 30oC. The proline content of irradiated Ubi Kuning was high when they were grown under 25 oC and 30oC, implying that these plantlets had the possibility to tolerant to lower and higher-temperature condition. This study is initially useful to find out the growth ability of irradiated Ubi Kuning in response to lower and higher temperature.
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