Morphological and Histological Study of White Rats (Rattus norvegicus) Kidney Following the Consumption of Sumatran Wild Mango Extract (Mangifera spp.)

The development of medicine requires preclinical testing of experimental animals. One of the potential plants to be developed as a new medicinal source is Sumatran wild mangoes (Mangifera spp.) which are known to have immunomodulatory activity and higher antioxidant value than cultivated Mangifera. This study was aimed to test the sub-chronic toxicity of the extract of 6 wild mango species on white rats’ kidney function. This study used 21 treatments and 3 replications, consisted of 3 control treatments (control 0, with distilled water, positive control (stimuno) and negative control (CMC-Na 1%), and 6 types of Mangifera spp. (Mangifera sp.2 (Hutan), M. sumatrana, M. foetida (var. Batu, Limus, and Manis), and M. laurina) with 3 different dosages. Observations were made macroscopically (morphology) and histology through the glomerulus image to see the percentage of damage. The percentage of glomerulus damage was analyzed by ANOVA and Duncan's test. Based on the results showed that the kidney morphology looked like a pea with brownish-red color and the capsule looked flat (healthy) after being treated. Histologically, the administration of wild mango extract did not cause damage to the rats' kidneys, which was indicated by the glomerulus which was still covered by the bowman capsule and the boundary between the pars visceral epithelium and pars parietal epithelium was clearly visible.