The Effect of Exercise and Folate Nano-Liposomes on D1 and D2 Receptor Gene Expression in the Brain of Alzheimer's Rats

Background and objectives: Alzheimer's is a neurodegenerative disorder characterized by memory loss and cognitive dysfunction. Research has shown that blood metal levels and physical activity may be crucial to injury and possibly Alzheimer's treatment. This study aimed to evaluate the effects of high-intensity interval training (HIIT) and folate Nano liposomes on the expression of D1 and D2 genes in the hippocampal tissue of Alzheimer's rats. Material and Methods: Thirty-five male Wistar rats at eight weeks of age were randomly divided into five groups (healthy control, Alzheimer's control, Alzheimer's + HIIT, Alzheimer's + Nano-liposome folate, Alzheimer's + HIIT + Nano-liposome folate). After Alzheimer's induction, exercise program protocol and folate Nano liposomes were performed as supplements in the groups. After the last training session, the mice were anesthetized, and the hippocampus was examined. D1 and D2 receptor gene expression were examined by the Real-time-PCR method. Results: The results showed that the highest increase in D1 and D2 receptor gene expression was in the Alzheimer's group, and the lowest growth was in the Alzheimer's+HIIT+ Nano liposome group (P<0.05). Conclusion: Resistance training and Nano-liposome folate decreased D1 and D2 receptor gene expression after Alzheimer's induction. This reduction may be due to the modulatory effects of physical activity and Nanomedicines in preventing or reducing pathological conditions.