急性肠系膜缺血/再灌注下调肾PGE2合成。

IF 2.9 4区医学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Prostaglandins, leukotrienes, and essential fatty acids Pub Date : 1994-01-01 DOI:10.1097/00024382-199401001-00178

S. Myers, R. Hernandez, J. Horton

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引用次数: 0

摘要

本研究探讨了己酮茶碱在肠系膜缺血/再灌注损伤中保护肾脏PGE2合成的假设。麻醉后的Sprague-Dawley大鼠(300 g)假手术或肠系膜上动脉阻断20 min，再灌注30 min。缺血/再灌注组在缺血前5天每天给予别嘌呤醇(10 mg/kg)，缺血前10分钟给予己酮茶碱(50 mg/kg)或载体。取肾，体外充氧Krebs缓冲液灌注，用酶免疫法测定排出液中6-酮- pgf1 α、PGE2和血栓素B2 (TXB2)的释放。肠系膜缺血/再灌注使肾脏PGE2释放减少50%(与假手术相比)，但不改变TXB2或6-酮- pgf1 α的释放。己酮茶碱预处理(而不是别嘌呤醇)在假手术水平上保留了肾脏PGE2的释放。这些数据表明，己酮茶碱通过维持肾PGE2(一种有效的内源性肾血管扩张剂)的释放，对严重的肠系膜缺血/再灌注损伤具有保护作用。

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Acute mesenteric ischemia/reperfusion down regulates renal PGE2 synthesis.

This study examines the hypothesis that pentoxifylline protects renal PGE2 synthesis during mesenteric ischemia/reperfusion injury. Anesthetized Sprague-Dawley rats (300 g) were subjected to sham or superior mesenteric artery occlusion for 20 min followed by 30 min of reperfusion. The ischemia/reperfusion groups received either enteral allopurinol (10 mg/kg) daily for 5 d prior to ischemia, pentoxifylline (50 mg/kg) 10 min prior to ischemia or carrier. The kidney was removed and perfused in vitro with oxygenated Krebs buffer and the effluent was assayed for release of 6-keto-PGF1 alpha, PGE2 and thromboxane B2 (TXB2) by enzyme immunoassay. Mesenteric ischemia/reperfusion decreased renal PGE2 release by 50% (compared to sham) but did not alter release of TXB2 or 6-keto-PGF1 alpha. Pentoxifylline pretreatment (not allopurinol) preserved renal PGE2 release at the sham level. These data showed pentoxifylline exerted a protective effect against severe mesenteric ischemia/reperfusion injury by maintaining release of renal PGE2, a potent endogenous renal vasodilator.

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来源期刊

Prostaglandins, leukotrienes, and essential fatty acids 医学-内分泌学与代谢

CiteScore

6.40

自引率

6.70%

发文量

审稿时长

13.2 weeks

期刊介绍： The role of lipids, including essential fatty acids and their prostaglandin, leukotriene and other derivatives, is now evident in almost all areas of biomedical science. Cell membrane behaviour and cell signalling in all tissues are highly dependent on the lipid constituents of cells. Prostaglandins, Leukotrienes & Essential Fatty Acids aims to cover all aspects of the roles of lipids in cellular, organ and whole organism function, and places a particular emphasis on human studies. Papers concerning all medical specialties are published. Much of the material is particularly relevant to the development of novel treatments for disease.