非小细胞肺癌患者信号转导蛋白的表达及其与临床表现的关系

Dennie V. Jones, Souzan Sanati, A. Haque, D. Freeman, I. Kessel, J. Zwischenberger, Jingwu Xie
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引用次数: 0

摘要

在非小细胞肺癌(NSCLC)中,预后因素已经确定,包括肿瘤分期和表现状态,然而,同一阶段的生存是可变的,需要额外的预后因素。我们研究了108例非小细胞肺癌石蜡包埋切片的微阵列,这些切片用Ki-67、FRAP、HER2、PTEN、VEGF、COX-2、CD117和PDGF单克隆抗体免疫染色,采用增强敏感性的亲和素-生物素过氧化物酶技术。染色按阳性细胞的百分比进行分级,结果通过log-rank检验和Kaplan Meier生存图与存活率相关。生存率的提高与女性、鳞状细胞组织学、低Ki67表达和缺乏FRAP表达有关。VEGF和CD117的表达与分期相关,而COX2的表达在种族间存在显著差异。HER2、PTEN和PDGF的染色模式在病理因素、患者人口统计学或生存结果方面没有统计学差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Expression Of Signal Transduction Proteins And Their Relationship To Clinical Findings In Patients With Nonsmall Cell Lung Cancer
In nonsmall cell lung cancer (NSCLC), prognostic factors have been established including tumor stage and performance status, however, survival within the same stage is variable and additional prognostic factors are needed. We investigated a microarray of paraffin-embedded sections from 108 cases of NSCLC which were immunostained with monoclonal antibodies against Ki-67, FRAP, HER2, PTEN, VEGF, COX-2, CD117, and PDGF, using an enhanced sensitivity avidin-biotin peroxidase technique. Staining was graded as a percentage of positive cells, and results correlated with survival by log-rank test and Kaplan Meier survival plots. Improved survival was correlated with female gender, squamous cell histology, low Ki67 expression, and lack of FRAP expression. VEGF and CD117 expression was correlated with stage, while COX2 expression showed significant differences by ethnicity. There was no statistical difference between the staining patterns of HER2, PTEN, and PDGF by pathologic factor, patient demographics, or survival outcome.
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