从尼日利亚河流州哈科特港出售的蜗牛(Archachatina marginata)中分离出的沙门氏菌的流行率和抗生素谱

V. Daminabo, D. Ogbonna, N. Odu, Lawrence O. Amadi
{"title":"从尼日利亚河流州哈科特港出售的蜗牛(Archachatina marginata)中分离出的沙门氏菌的流行率和抗生素谱","authors":"V. Daminabo, D. Ogbonna, N. Odu, Lawrence O. Amadi","doi":"10.9734/EJNFS/2020/V12I1230336","DOIUrl":null,"url":null,"abstract":"Increase in microbial population especially Salmonella species in food due to improper handling, storage and exposure to contaminants can raise public health concerns when consumed without adequate processing. This study evaluates the prevalence and antibiogram of Salmonella species associated with the giant land Snail (Archachatina marginata) sold in markets around Port Harcourt metropolis. A total number of seventy two (72) samples of land snail were collected from three markets; Creek Road, Mile one and Rumuokoro. The samples were labelled and transported in an ice packed coolers to the laboratory for analyses. Standard microbiological protocols were employed to determine the microbial load and species of the various parts (intestine, meat and fluid) of the snail samples after shucking. Antibiotics sensitivity profile testing of the isolated and identified Salmonella species were carried out as recommended by Clinical Laboratory Standard Institute (CLSI) and statistical analyses using one way ANOVA and all pairs Turkey-Kramer. Results from the study showed that the highest total heterotrophic bacteria count (THBC) of 8.6x106CFU/g was obtained in the snail intestine sourced from Creek road market while THBCs  of 8.2x106 CFU/g and 7.3x106 CFU/g were from Mile one and Rumuokoro markets respectively. THBCs of meat from the markets ranged from 4.3-5.4x106 CFU/g and 3.7-4.9x106CFU/ml in fluid with Rumuokoro having the least occurrence respectively. Mean Salmonella counts (MSCs) ranged from 0.4-3.6 x103 CFU/g, with least count obtained from Rumuokoro and Mile 1 markets. Similarly, least MSCs in fluid and intestine were obtained from Rumuokoro and Mile 1 whereas Creek road Market had the highest respectively. Three species of Salmonella; S. arizonae, S. gallinarum and S. typhi were predominant in addition to other species such as Vibrio spp,, Bacillus spp., Staphylococcus spp., Shigella spp., Pseudomonas spp., Enterobacter spp., E. coli, Micrococcus spp., Acinetobacter spp., Klebsiella spp. and Listeria spp identified using both conventional and molecular method. Antibiogram profile revealed that all the identified Salmonella species were susceptible to Ofloxacin and Ciprofloxacin but strongly resistance to Cetazidime and Gentamicin. The diversity and elevated microbial load observed from this study calls for caution in handling and processing of snails since most of these bacteria may become aetiologic agents of several food-borne diseases and other pathological conditions. As a necessity, good quality control measures and proper chemotherapy should be administered to patients with signs and symptoms of food borne illness emanating from consumption of snail.","PeriodicalId":11994,"journal":{"name":"European Journal of Nutrition & Food Safety","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence and Antibiogram of Salmonella Species Isolated from Snail (Archachatina marginata) Sold in Port Harcourt, Rivers State, Nigeria\",\"authors\":\"V. Daminabo, D. Ogbonna, N. Odu, Lawrence O. Amadi\",\"doi\":\"10.9734/EJNFS/2020/V12I1230336\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Increase in microbial population especially Salmonella species in food due to improper handling, storage and exposure to contaminants can raise public health concerns when consumed without adequate processing. This study evaluates the prevalence and antibiogram of Salmonella species associated with the giant land Snail (Archachatina marginata) sold in markets around Port Harcourt metropolis. A total number of seventy two (72) samples of land snail were collected from three markets; Creek Road, Mile one and Rumuokoro. The samples were labelled and transported in an ice packed coolers to the laboratory for analyses. Standard microbiological protocols were employed to determine the microbial load and species of the various parts (intestine, meat and fluid) of the snail samples after shucking. Antibiotics sensitivity profile testing of the isolated and identified Salmonella species were carried out as recommended by Clinical Laboratory Standard Institute (CLSI) and statistical analyses using one way ANOVA and all pairs Turkey-Kramer. Results from the study showed that the highest total heterotrophic bacteria count (THBC) of 8.6x106CFU/g was obtained in the snail intestine sourced from Creek road market while THBCs  of 8.2x106 CFU/g and 7.3x106 CFU/g were from Mile one and Rumuokoro markets respectively. THBCs of meat from the markets ranged from 4.3-5.4x106 CFU/g and 3.7-4.9x106CFU/ml in fluid with Rumuokoro having the least occurrence respectively. Mean Salmonella counts (MSCs) ranged from 0.4-3.6 x103 CFU/g, with least count obtained from Rumuokoro and Mile 1 markets. Similarly, least MSCs in fluid and intestine were obtained from Rumuokoro and Mile 1 whereas Creek road Market had the highest respectively. Three species of Salmonella; S. arizonae, S. gallinarum and S. typhi were predominant in addition to other species such as Vibrio spp,, Bacillus spp., Staphylococcus spp., Shigella spp., Pseudomonas spp., Enterobacter spp., E. coli, Micrococcus spp., Acinetobacter spp., Klebsiella spp. and Listeria spp identified using both conventional and molecular method. Antibiogram profile revealed that all the identified Salmonella species were susceptible to Ofloxacin and Ciprofloxacin but strongly resistance to Cetazidime and Gentamicin. The diversity and elevated microbial load observed from this study calls for caution in handling and processing of snails since most of these bacteria may become aetiologic agents of several food-borne diseases and other pathological conditions. As a necessity, good quality control measures and proper chemotherapy should be administered to patients with signs and symptoms of food borne illness emanating from consumption of snail.\",\"PeriodicalId\":11994,\"journal\":{\"name\":\"European Journal of Nutrition & Food Safety\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Journal of Nutrition & Food Safety\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9734/EJNFS/2020/V12I1230336\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Nutrition & Food Safety","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/EJNFS/2020/V12I1230336","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

由于处理、储存不当和接触污染物而导致食物中微生物数量增加,特别是沙门氏菌种类增加,在未经适当处理的情况下食用会引起公众健康问题。本研究评估了在哈科特港大都会市场销售的巨型地螺(Archachatina marginata)相关沙门氏菌的流行率和抗生素谱。在3个市场共采集72只钉螺样本;克里克路,一英里路和鲁穆科罗路。这些样品被贴上标签,用冰包装的冷却器运送到实验室进行分析。采用标准微生物学方案测定脱壳后蜗牛样品各部分(肠、肉和体液)的微生物负荷和种类。按照临床实验室标准协会(CLSI)的建议,对分离和鉴定的沙门氏菌进行抗生素敏感性测试,并采用单因素方差分析和全对Turkey-Kramer进行统计分析。结果表明,来自溪路市场的螺肠总异养菌数(THBC)最高,为8.6x106CFU/g,而来自Mile one和Rumuokoro市场的THBC分别为8.2x106 CFU/g和7.3x106 CFU/g。市场肉类的四氢溴化物含量为4.3-5.4 × 106CFU/ g,液体中为3.7-4.9 × 106cfu /ml,其中Rumuokoro的发生率最低。平均沙门氏菌计数(MSCs)范围为0.4-3.6 x103 CFU/g, Rumuokoro和Mile 1市场的沙门氏菌计数最少。同样,Rumuokoro和Mile 1市场的液体和肠道中MSCs含量最少,而Creek road Market市场的MSCs含量最高。三种沙门氏菌;常规方法和分子方法检出的主要菌种有:弧菌、芽孢杆菌、葡萄球菌、志贺氏菌、假单胞菌、肠杆菌、大肠杆菌、微球菌、不动杆菌、克雷伯氏菌和李斯特菌。抗生素谱分析显示,所有沙门氏菌对氧氟沙星和环丙沙星敏感,对西他啶和庆大霉素耐药。从本研究中观察到的多样性和增加的微生物负荷要求在处理和加工蜗牛时要谨慎,因为大多数这些细菌可能成为几种食源性疾病和其他病理状况的病原。对于因食用蜗牛而引起食源性疾病的患者,有必要采取良好的质量控制措施和适当的化疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prevalence and Antibiogram of Salmonella Species Isolated from Snail (Archachatina marginata) Sold in Port Harcourt, Rivers State, Nigeria
Increase in microbial population especially Salmonella species in food due to improper handling, storage and exposure to contaminants can raise public health concerns when consumed without adequate processing. This study evaluates the prevalence and antibiogram of Salmonella species associated with the giant land Snail (Archachatina marginata) sold in markets around Port Harcourt metropolis. A total number of seventy two (72) samples of land snail were collected from three markets; Creek Road, Mile one and Rumuokoro. The samples were labelled and transported in an ice packed coolers to the laboratory for analyses. Standard microbiological protocols were employed to determine the microbial load and species of the various parts (intestine, meat and fluid) of the snail samples after shucking. Antibiotics sensitivity profile testing of the isolated and identified Salmonella species were carried out as recommended by Clinical Laboratory Standard Institute (CLSI) and statistical analyses using one way ANOVA and all pairs Turkey-Kramer. Results from the study showed that the highest total heterotrophic bacteria count (THBC) of 8.6x106CFU/g was obtained in the snail intestine sourced from Creek road market while THBCs  of 8.2x106 CFU/g and 7.3x106 CFU/g were from Mile one and Rumuokoro markets respectively. THBCs of meat from the markets ranged from 4.3-5.4x106 CFU/g and 3.7-4.9x106CFU/ml in fluid with Rumuokoro having the least occurrence respectively. Mean Salmonella counts (MSCs) ranged from 0.4-3.6 x103 CFU/g, with least count obtained from Rumuokoro and Mile 1 markets. Similarly, least MSCs in fluid and intestine were obtained from Rumuokoro and Mile 1 whereas Creek road Market had the highest respectively. Three species of Salmonella; S. arizonae, S. gallinarum and S. typhi were predominant in addition to other species such as Vibrio spp,, Bacillus spp., Staphylococcus spp., Shigella spp., Pseudomonas spp., Enterobacter spp., E. coli, Micrococcus spp., Acinetobacter spp., Klebsiella spp. and Listeria spp identified using both conventional and molecular method. Antibiogram profile revealed that all the identified Salmonella species were susceptible to Ofloxacin and Ciprofloxacin but strongly resistance to Cetazidime and Gentamicin. The diversity and elevated microbial load observed from this study calls for caution in handling and processing of snails since most of these bacteria may become aetiologic agents of several food-borne diseases and other pathological conditions. As a necessity, good quality control measures and proper chemotherapy should be administered to patients with signs and symptoms of food borne illness emanating from consumption of snail.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信