叙利亚杏果实中类胡萝卜素最佳提取条件的确定

Wahbi Kalook, A. Faleh, A. Sakur, W. Abdelwahed
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引用次数: 1

摘要

本研究的目的是利用食品溶剂(乙醇)和非食品溶剂(丙醇)从杏果实中提取类胡萝卜素。此外,研究不同提取条件,即提取温度(20-40-60)℃和提取时间(4-8-12)h对类胡萝卜素得率的影响,以改进和发展提取方法。采用高效液相色谱法对提取的类胡萝卜素进行测定,所研究的类胡萝卜素为α-胡萝卜素和β-胡萝卜素。研究发现,杏中β-胡萝卜素约占类胡萝卜素总量的80%。结果表明,乙醇萃取比丙醇萃取得率低。与乙醇相比,丙醇是最适合提取类胡萝卜素的溶剂。温度对提取率也有显著影响;在20℃下,胡萝卜素的提取率很低,60℃为提取胡萝卜素的最佳温度。在提取的第一个小时,提取率随时间的增加而显著增加,而在6-8小时内,增加提取时间对提取率的影响不显著。此外,水果冷冻预处理加速了提取过程,提高了提取率。在食品提取条件下,确定了类胡萝卜素的最佳提取条件;提取类胡萝卜素的最佳条件为:以乙醇为食品溶剂,在60℃条件下提取3小时,冷冻预处理,提取率可达6.36 mg/100 g。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determining the Optimum Conditions for Extracting Carotenoids from Syrian Apricot Fruits
The aim of this research is to extract carotenoids from apricot fruits using a food solvent (ethanol) and a non-food solvent (propanol). In addition, it aims to study the effect of different extraction conditions, i.e., the extraction temperature (20-40-60)°C and the extraction times (4-8-12) hours, on the carotenoids yield in order to improve and develop extraction methods. The extracted carotenoids were determined using high-performance liquid chromatography (HPLC), and the studied carotenoids are α-carotene and β-carotene. It was found that β-carotene constitutes about 80% of the total carotenoids in apricots. The results indicated that the extraction yield using ethanol was low compared to the extraction yield using propanol. Propanol is the most suitable solvent for carotenoids extraction in comparison with ethanol. The temperature also had a significant effect on the extraction yield; at 20°C the extraction yield was very low, and 60°C was the optimum temperature for the extraction of carotene. The extraction yield significantly increased with time in the first hours of extraction, and there was no significant effect from increasing the extraction time for a period of 6-8 hours. Moreover, the pretreatment of fruits by freezing accelerated the extraction process and increased the extraction yield. The optimum conditions for extracting carotenoids were determined in the conditions of food extraction; the optimum conditions for extracting carotenoids are: extraction at 60°C for three hours with pretreatment by freezing as the extraction rate reached up to 6.36 mg/100 g using ethanol as a food solvent.
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