磁电泳细胞术定量测定细胞表面表达

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII) Pub Date : 2019-06-23 DOI:10.1109/TRANSDUCERS.2019.8808437

Ozgun Civelekoglu, Ningquan Wang, Mert Boya, Tevhide Ozkaya-Ahmadov, Ruxiu Liu, A. F. Sarioglu

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引用次数: 1

摘要

膜抗原的鉴定和测定其在细胞群中的表达对医学和生物学研究具有重要意义。在这项工作中，我们提出了一种在微流体系统中基于磁泳术和分布式库尔特传感的细胞计数方法。与复杂、昂贵和大型系统的传统流式细胞仪相比，我们的磁电泳细胞仪在便携式和一次性平台上提供细胞膜抗原的定量分析。我们对人类乳腺癌细胞的测试显示了我们的微流体装置的实用性及其作为生物医学测试的即时护理仪器的潜力。

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Quantitative Measurement of Cell Surface Expression Via Magnetophoretic Cytometry

Identification of membrane antigens and measurement of their expression within a cell population is of fundamental importance to medical and biological studies. In this work, we present a cytometry approach that is based on magnetophoresis and distributed Coulter sensing in a microfluidic system. Our magnetophoretic cytometer offers quantitative analysis of cell membrane antigens on a portable and disposable platform compared to conventional flow cytometers, which are complex, expensive and large systems. Our tests with human breast cancer cells show the utility of our microfluidic device and its potential as a point-of-care instrument for biomedical testing.

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来源期刊

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)

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