대학 합성생물학 실습수업용 프로그램 개발 - 유전자 회로 조립 실험과 빛 테두리 그림 그리기 실험을 바탕으로 -

The purpose of this study is to develop a practical training program for synthetic biology that introduces biology major students to the field of synthetic biology and enables students to experience an exciting part of the synthetic biology research. This program consists of two hands-on modules. The first module constructs a gene circuit that produces red fluorescent proteins, allowing students to observe the result directly through their eyes. In the second module, students learn common molecular logic of gene circuits by practicing bacterial programming that enables bacteria to detect the light border. This practice program was applied to the biology major students, and their perception of the contents of the program was measured. Learning assessment quizzes revealed that ~87%(7/8) of the participants well understood the key concepts in this practice program. In addition, all participants, except one, responded that the experimental design to visually check whether a genetic circuit works increased their interest in the field of synthetic biology. The results show the possibility of this program as an introductory practice course for undergraduate synthetic biology education.