Effect of Chia Seed Extract (Salvia Hispanica L) On Current Blood Sugar Levels and MDA Levels

Hyperglycemia is a condition in which the body experiences an increase in blood sugar levels and is a clinical manifestation of type 2 DM which will increase ROS so that an antioxidant imbalance occurs which results in oxidative stress, one of the biomarkers, namely MDA. Chia seeds contain high antioxidants so that they are effective in reducing oxidative stress caused by hyperglycemia in type 2 diabetes. The research study was to prove the effect of chia seed extract on GDS and MDA levels in DMT2 rats with STZ injection. The metods is experimental research with Posttest Only Control Group Design. The number of samples was 25 male wistar rats aged 3-4 months with a weight of 180-200 grams and adapted for 7 days, divided into 5 groups randomly, namely the treatment group P0 as a control, P1 as a positive control, P2 as a negative control, and P3 as a control group. STZ injection and chia seed extract at a dose of 4.5 mg/KgBW/day, P4 for STZ injection and chia seed extract at a dose of 13.5 mg/KgBW/day. Treatment time is 7 days. Blood samples were taken from v. Ophtalmica on day 14. Examination of GDS levels using the ELISA method and examination of MDA levels using the TBARS method, the data were analyzed by the Anova test. The result Mean level of GDS P0: 127.6±2.8; P1: 204.6±6.1; P2: 76.2±1.3; P3: 45.6±2.07; P4: 40±1.58. Mean levels of MDA in nmol/ml P0: 0.93±0.04; P1: 1.20±0.01; P2: 0.73±0.04; P3: 0.44±0.03; P4: 0.22±0.20. The results of the ANOVA test obtained a P value of 0.000 and the results of the post hoc LSD test between groups obtained a P value of 0.000 (<0.05). conclusion is significant difference in reducing GDS and MDA levels between Chia seed extract doses of 4.5 mg/KgBW/day and 13.5 mg/kgBW/day. Keywords: chia seeds; MDA; GDS; DM Type 2