{"title":"三相分割法纯化苦瓜转化酶的研究","authors":"Nihan Kübra Belligün, B. Demir","doi":"10.5897/AJBR2019.1033","DOIUrl":null,"url":null,"abstract":"The single step three-phase partitioning (TPP) method was evaluated for the purification of invertase from Momordica charantia. Optimum conditions for TPP method, that is, 70% ammonium sulfate saturation, 1:0.5 t-butanol ratio and 30 min incubation time lead to 20.28% efficiency and 10.48 fold partial purification. Total protein decreased from 34.86 mg to 0.68 mg in the homogenate, and the specific activity of the enzyme increased from 21.67 to 227.02 U/mg protein. The purified enzyme showed maximum activity in 0.3 M pH 5.0 buffer at 50°C. In conclusion, the optimized single step TPP method revealed better purification. \n \n \n \n Key words: Three-phase partitioning (TPP), invertase, Momordica charantia, enzyme purification.","PeriodicalId":7631,"journal":{"name":"African Journal of Biochemistry Research","volume":"39 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Partial purification of invertase from Momordica charantia (bitter melon) by three phase partitioning (TPP) method\",\"authors\":\"Nihan Kübra Belligün, B. Demir\",\"doi\":\"10.5897/AJBR2019.1033\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The single step three-phase partitioning (TPP) method was evaluated for the purification of invertase from Momordica charantia. Optimum conditions for TPP method, that is, 70% ammonium sulfate saturation, 1:0.5 t-butanol ratio and 30 min incubation time lead to 20.28% efficiency and 10.48 fold partial purification. Total protein decreased from 34.86 mg to 0.68 mg in the homogenate, and the specific activity of the enzyme increased from 21.67 to 227.02 U/mg protein. The purified enzyme showed maximum activity in 0.3 M pH 5.0 buffer at 50°C. In conclusion, the optimized single step TPP method revealed better purification. \\n \\n \\n \\n Key words: Three-phase partitioning (TPP), invertase, Momordica charantia, enzyme purification.\",\"PeriodicalId\":7631,\"journal\":{\"name\":\"African Journal of Biochemistry Research\",\"volume\":\"39 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"African Journal of Biochemistry Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5897/AJBR2019.1033\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"African Journal of Biochemistry Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5897/AJBR2019.1033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
摘要
对单步三相分割法纯化苦瓜转化酶进行了研究。TPP法的最佳工艺条件为硫酸铵饱和度70%，丁醇比1:0.5，培养时间30 min，效率20.28%，部分纯化率10.48倍。匀浆中总蛋白从34.86 mg降低到0.68 mg，酶比活性从21.67 U/mg蛋白提高到227.02 U/mg蛋白。纯化后的酶在0.3 M pH 5.0的缓冲液中在50°C下表现出最大的活性。综上所述，优化后的单步TPP法纯化效果较好。关键词:三相分割，转化酶，苦瓜，酶纯化
Partial purification of invertase from Momordica charantia (bitter melon) by three phase partitioning (TPP) method
The single step three-phase partitioning (TPP) method was evaluated for the purification of invertase from Momordica charantia. Optimum conditions for TPP method, that is, 70% ammonium sulfate saturation, 1:0.5 t-butanol ratio and 30 min incubation time lead to 20.28% efficiency and 10.48 fold partial purification. Total protein decreased from 34.86 mg to 0.68 mg in the homogenate, and the specific activity of the enzyme increased from 21.67 to 227.02 U/mg protein. The purified enzyme showed maximum activity in 0.3 M pH 5.0 buffer at 50°C. In conclusion, the optimized single step TPP method revealed better purification.
Key words: Three-phase partitioning (TPP), invertase, Momordica charantia, enzyme purification.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
